朱芳艳等
摘要[目的]研究不同浓度NaCl和PEG6000对高雪轮、矮雪轮、五彩石竹、须苞石竹、麦仙翁和花园鼠尾草6种花卉种子萌发的影响,了解6种花卉种子对盐碱和干旱的适应性。[方法]对6种花卉种子进行不同浓度NaCl和PEG 6000胁迫处理,研究6种花卉种子在胁迫下的萌发特性。[结果]当NaCl浓度大于200 mmol/L时,明显抑制了高雪轮和矮雪轮种子的萌发;浓度大于300 mmol/L时,明显抑制麦仙翁、五彩石竹、须苞石竹和花园鼠尾草种子的萌发。当PEG6000浓度大于5%时,明显抑制花园鼠尾草种子的萌发;浓度大于20%时,明显抑制高雪轮种子的萌发;浓度大于25%时,明显抑制麦仙翁、须苞石竹、五彩石竹和矮雪轮种子的萌发。[结论] 6种花卉种子均可耐重度盐胁迫,麦仙翁、五彩石竹、须苞石竹和花园鼠尾草种子的耐盐性更强;麦仙翁、须苞石竹、五彩石竹和矮雪轮种子的耐旱性强,高雪轮种子的耐旱性较强,花园鼠尾草种子的耐旱性差。
关键词NaCl;PEG6000;胁迫;花卉种子;萌发
中图分类号S604+文献标识码A文章编号0517-6611(2014)21-06948-03
Effects of NACL and PEG on Seeds Germination of Six Flowers
ZHU Fangyan, GUO Jinli et al(College of Agronomy, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019)
Abstract[Objective] To study the effects of different concentration NaCl and PEG6000 on seeds germination of Silene pendula, Silene armeria, Agrostemma githago, Dianthus chinensis, Salvia farinacea, Dianthus chinensis, and obtain the adaptability of six flower seeds to salt and drought. [Method] Different concentrations of NaCl and PEG 6000 stress treatment were conducted on 6 flower seeds, the germination characteristics were studied. [Result] The results showed that: when NaCl solution concentration was more than 200 mmol/L, it inhibited the seed germination significantly in Silene pendula and Silene armeria; when the concentration was greater than 300 mmol/L, it significantly inhibited the seed germination in Agrostemma githago, Dianthus chinensis, Salvia farinacea, and Dianthus chinensis. When the solution concentration of PEG6000 was greater than 5%, it inhibited the seed germination of Dianthus chinensis; when the solution concentration was greater than 20%, it inhibited the seed germination in Silene armeria; when the solution concentration was greater than 25%, it inhibited the seed germination in Agrostemma githago, Dianthus chinensis, Salvia farinacea, and Silene pendula. [Conclusion] Seeds of Agrostemma githago, Dianthus chinensis, Salvia farinacea, Dianthus barbatus have stronger salt tolerance ability; seeds of Silene pendula, Agrostemma githago, Dianthus chinensis, Dianthus barbatus have the strongest drought tolerance ability, seeds of Silene armeria take the second place, and seeds of Salvia farinacea are the poorest.
Key wordsNaCl; PEG6000; Stress; Flower seeds; Germination
收稿日期20140620种子萌发是植物生活史中的重要阶段[1]。在种子萌发时,干旱和盐分是影响植物种子萌发的关键性因素,决定着一些植物能否成功在干旱、盐渍环境中定居[2-4],了解花卉种子对干旱和盐分土地的适应能力对于植物的生存具有重要意义。为此,笔者研究不同浓度NaCl和PEG6000溶液对矮雪轮(Silene pendula)、高雪輪(Silene armeria)、麦仙翁(Agrostemma githago L.)、五彩石竹(Dianthus Chinensis)、须苞石竹(Dianthus barbatus)和花园鼠尾草(Salvia farinacea)种子萌发的影响,了解6种花卉种子在不同干旱和盐胁迫下的适应能力,为生产实践提供理论依据。
1材料与方法
将6种花卉种子经浓度为0.3%高锰酸钾溶液消毒15 min,去离子水冲洗3~5次,分别放入盛有不同浓度胁迫溶液的培养皿中,每处理挑选饱满种子各30粒,3次重复。将培养皿置于ZPG280型智能光照培养箱中进行种子萌发,15 ℃恒温,矮雪轮光照条件为每天12 h光照,其他5种花卉种子光照條件为每天0 h光照。每天记载种子萌发情况及发芽粒数(以胚根突破种皮为萌发),
2结果与分析
2.1NaCl胁迫对6种花卉种子萌发的影响从表1可以看出,不同浓度NaCl对6种花卉种子的萌发均产生了不同程度的抑制作用,基本上随着浓度的增加抑制作用越明显。当NaCl浓度为100 mmol/L时,高雪轮和矮雪轮花卉种子的发芽率和对照相比无显著差异,矮雪轮种子的发芽势明显高于对照,高雪轮种子的发芽势和发芽指数明显低于对照;综上,当PEG6000浓度大于5%时明显抑制花园鼠尾草种子的萌发;浓度大于20%时,明显抑制了高雪轮种子的萌发;浓度大于25%时,明显抑制了麦仙翁、须苞石竹、五彩石竹和矮雪轮种子的萌发。
3讨论与结论
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