Effect of laparoscopic sleeve gastrectomy on related variables of obesity complicated with polycystic ovary syndrome

Xiao-Tao Wang,Yi-Sen Hou,Hao-Liang Zhao,Jian Wang,Chen-Hao Guo,Jie Guan,Zhi-Gan Lv,Peng Ma,Jian-Li Han

Abstract BACKGROUND Polycystic ovary syndrome (PCOS) is closely related to obesity,and weight loss can significantly improve the metabolic,endocrine and reproductive functions of obese individuals with PCOS.However,the efficacy of laparoscopic sleeve gastrectomy (LSG) for obesity with PCOS are unclear.AIM The purpose of the study was to investigate the effect of LSG on related variables in obese patients with PCOS.METHODS A retrospective analysis was performed on 32 obese patients with PCOS who received LSG treatment at the Third Hospital of Shanxi Medical University from 2013 to 2020.The changes in anthropometric indices,insulin,testosterone,estradiol,follicle stimulating hormone (FSH),luteinizing hormone (LH),menstrual cycle and LH/FSH ratio before and 1 mo,3 mo,6 mo and 12 mo after the operation were statistically analyzed.RESULTS At 1 mo,3 mo,6 mo and 12 mo after surgery,the anthropometric indices,such as body weight and body mass index,of all patients were lower than those before the operation.The percentage excess weight loss (EWL%) at 1 mo,3 mo,6 mo and 1 year of follow-up were 25,40,46 and 65,respectively.The PCOS-related indices,such as insulin,testosterone,estradiol,follicle stimulating hormone (FSH),luteinizing hormone (LH) and menstrual cycle,were improved to varying degrees.During the 1-year follow-up,the average serum testosterone decreased from preoperative 0.72 ng/mL to 0.43 ng/mL (P ＜ 0.05),average fasting insulin level (9.0 mIU/mL,preoperative 34.2 mil,LH level,4.4 mIU/mL,preoperative 6.1 mIU/mL).The level of FSH (3.8 U/L,4.8 U/p0.05) and the ratio of LH/FSH (0.7,1.3/p0.05) were more relieved than those before surgery.During the postoperative follow-up,it was found that the menstrual cycle of 27 patients (nasty 27) returned to normal,and 6 patients (18%) who intended to become pregnant became pregnant within 1 year after surgery.CONCLUSION The weight loss effect of LSG is obvious and affirmative,and the endocrine index of obese patients with PCOS is also improved to some extent,although the mechanism is not clear.Laparoscopic sleeve gastrectomy is expected to become a backup choice for patients with polycystic ovaries in the future.

Key Words: Laparoscopic sleeve gastrectomy;Polycystic ovary syndrome;Hyperandrogenism;Insulin resistance

INTRODUCTION

The World Health Organization (WHO) confirms that obesity is a 21stcentury epidemic that can lead to hormonal imbalances,such as polycystic ovary syndrome (PCOS)[1-3].PCOS is a common endocrine disease in young women,with a prevalence rate of 5%-18%[4-5].The widely accepted mechanisms include insulin resistance leading to hyperinsulinemia,stimulating ovarian sheath cells to produce androgen,which is characterized by hypomenorrhea and hyperandrogenemia,and an increased risk of gynecological diseases such as hirsutism,infertility,and ovarian and endometrial cancer[6-8].The incidence of PCOS in obese women is as high as 50%.

PCOS is closely related to obesity,and an increase in obesity will enhance the severity and expression of the PCOS phenotype.Current data suggest that weight loss is associated with metabolic,endocrine and reproductive improvements in obese women with PCOS.In addition to significant weight loss,bariatric surgery can also lead to recovery of the hypothalamic-pituitary axis,normal menstruation,improvement of hirsutism,reduction of cardiovascular risk,and improvement of fertility[9].At present,the methods of weight loss are complex and diverse.The two most common types of bariatric surgery are laparoscopic sleeve gastrectomy (LSG) and laparoscopic Roux gastric bypass(LRYGB)[10].LRYGB is found to be superior in long-term remission of dyslipidemia and hypertension while LSG is relatively low difficulty,small changes to the original structure of the human body and few postoperative complications[11].Laparoscopic sleeve gastrectomy has been the most commonly performed bariatric procedure since 2014 and continues to steadily increase in number and percentage of all bariatric procedures year after year[12].In this study,the clinical data of 32 obese patients with PCOS after laparoscopic sleeve gastrectomy were retrospectively analyzed,and the changes in PCOS after laparoscopic sleeve gastrectomy were analyzed.

MATERIALS AND METHODS

General information

The clinical data of 32 patients who were diagnosed with obesity complicated with polycystic ovary syndrome and underwent laparoscopic sleeve gastrectomy in the Department of Weight Loss and Metabolic Surgery of the Third Hospital of Shanxi Medical University from January 2013 to December 2020 were reviewed.Body weight,fasting insulin,testosterone,estrogen,FSH,LH and other endocrine and metabolic indices were statistically analyzed before the operation and 1 mo,3 mo,6 mo and 12 mo after the operation.The inclusion criteria were as follows: (1) The diagnosis of obesity was clear,and there were surgical indications according to the guidelines;(2) according to the guidelines,the diagnosis of polycystic ovary syndrome was clear;(3) the patient signed informed consent for the operation and successfully completed laparoscopic sleeve gastrectomy;and (4) there were no postoperative complications or secondary operations.Exclusion criteria: (1) Polycystic ovary received surgical treatment or nearly 2 mo of treatment;(2) choose other methods of weight loss;and (3) morbid obesity.

Surgical method

After successful anesthesia,the supine split leg position was taken,the skin 1.0 cm was cut along the navel,the visible Trocar was punctured into the abdomen once,and 1.2 cm Trocar was placed on the right side of the umbilical 10 cm;the left clavicle line of 10 cm was disposed into 5 mm Trocar,and a Kirschner needle was placed under the xiphoid process to block the left lobe of the liver,and the omentum tissue was cut off along the great curvature of the stomach,close to the gastric wall,up to 1.5 cm on the left side of the His angle of the cardia,and down to the pyloric 4 cm;a gastric tube approximately 1.2 cm in diameter was placed through the mouth.Close to the pylorus 5 cm,close to the gastric tube,the gastric tissue on the large curved side was resected so that the residual gastric cavity was tubular at approximately 100 mL.Inverted thorns can be absorbed by purse sutures to strengthen the seromuscular layer.The specimen was removed,and it was confirmed that there was no blood oozing or bleeding.The abdomen was closed,and the operation was ended.

Observation index

Body weight-related parameters [body weight,body mass index (BMI),percentage excess weight loss (EWL%)] and polycystic ovary syndrome-related parameters [fasting insulin,testosterone,estrogen,FSH,LH,menstrual cycle and LH/FSH ratio] were measured before and 1,3,6 and 12 mo after the operation.

Statistical methods

SPSS 23.0 statistical software was used.The measurement data in accordance with the normal distribution are expressed as the mean ± standard deviation (mean ± SD),and the counting data are expressed asn(%).The related indices before and after the operation were compared by paired samplettests,andP＜ 0.05 was considered statistically significant.

RESULTS

Between January 2013 and December 2020,32 obese women with PCOS participated in the study and underwent surgery without intraoperative or postoperative complications.The baseline information of 32 patients were shown in Table 1.The average age of the sample was 33.78 ± 7.31 years old.The body weight and BMI decreased significantly at 1,3,6 and 12 mo after the operation (mean preoperative=41.22 kg/m2;postoperative mean=28.98 kg/m2).The patient's body mass and BMI were basically stable at 1 year after the operation.See Table 2 and Figure 1.

Figure 1 Changes of percentage excess weight loss after operation.

Table 1 Baseline information of 32 patients

During the postoperative period,the average level of estradiol increased (mean preoperatively=81.1 PG/dL;postoperative mean=89.2 pg/dL),and the difference was not statistically significant.The average serum testosterone decreased from 0.72 ng/mL to 0.43 ng/mL (P=0.05),and the insulin level decreased significantly after the operation(preoperative value=34.2 mIU/mL;postoperative insulin level=9.0 mIU/mL).After the operation,the level of FSH decreased (mean before the operation=4.8 U/L,average after the operation=3.8 U/L),and the difference was not statistically significant.At the same time,the level of LH decreased significantly after the operation (P＜ 0.05) (mean preoperative=6.1 U/L;postoperative mean=4.4 U/L).The average LH/FSH ratio decreased after the operation (1.3 before and after the operation).The preoperative average value was greater than 1,indicating that the level of LH was higher than that of FSH.On the other hand,after surgical treatment,it was found that the average ratio was less than 1,indicating that the average FSH level after bariatric surgery was higher than that of LH.The decrease in the LH/FSH ratio was statistically significant (P=0.05).The menstrual cycle of 27 patients (nasty 27,84%) returned to normal,and 6 patients (18%) who intended to become pregnant became pregnant within 1 year after the operation.See Table 3.

Table 3 Changes of polycystic ovary syndrome related indexes before and after operation

DISCUSSION

According to the clinical research and theoretical basis of PCOS for many years,China's Diagnostic Criteria of Polycystic Ovary Syndrome[13] points out that oligomenorrhea or amenorrhea is a necessary diagnostic condition.In addition,hyperandrogenaemia and/or clinical manifestations and ultrasound polycystic ovaries are nonessential conditions,and one of them can diagnose polycystic ovary syndrome[14].Clinical hyperandrogenism can be detected by the serum testosterone index.Normal serum testosterone in females is generally below 0.68 ng/L.If it exceeds 0.7 ng/L,it can be diagnosed as hyperandrogenemia.The symptoms can be characterized by exuberant hair growth,male characteristics,oligomenorrhea or amenorrhea.High serum androgen levels directly inhibit follicle stimulating hormone secretion and eventually lead to infertility.The indices of FSH and LH in patients with polycystic ovary syndrome are higher than normal to varying degrees.The ratio of LH/FSH can also be used as an important index for the diagnosis of polycystic ovary syndrome.The pathogenesis of PCOS is not clear,and genetic,environmental and other factors interact with each other.The widely accepted theory is that hyperandrogenemia and hyperinsulinemia caused by insulin resistance are its characteristics.In this study,insulin was also used as an important indicator to evaluate the remission of PCOS[15].

According to the diagnostic criteria of polycystic ovary syndrome,the testosterone index was used as the indication of hyperandrogenism,the insulin index was used as the manifestation of insulin resistance,and oligoovulation showed abnormal gonadotropin secretion,which could be observed by FSH and LH.The changes in related indices in patients with PCOS before and after the operation were analyzed.Anovulation in patients with PCOS is characterized byabnormal secretion of gonadotropin,which is usually characterized by increased serum LH concentration and normal or low FSH,resulting in an increase in the ratio of LH/FSH.Clinically,the ratio is used as an index to predict ovarian reserve function,and an increase in the ratio indicates a decrease in female ovarian reserve function[16].In this study,there were important changes in gonadotropin secretion after LSG;that is,FSH synthesis increased,and LH synthesis decreased,resulting in the reversal of the LH/FSH ratio.In this way,in addition to the reduction in plasma insulin,it helps to reduce hyperandrogenemia,promote the complete development of antral follicles and is conducive to ovulation.During the return visit,we found that some patients became pregnant within six months after the operation.This study did not evaluate the long-term effects of reproductive parameters such as infertility,but a systematic review published by Dağetal[17] showed that bariatric surgery can significantly improve menstrual irregularity and hirsutism,and fertility may be improved after bariatric surgery.The ovulation process leads to the formation of the corpus luteum and the continuous release of progesterone from the luteal structure,which reduces the risk of endometrial hyperplasia and cancer[13].Gonadotropin levels show hormonal oscillations during the menstrual cycle,which makes the study of gonadotropin more meaningful by taking the LH/FSH ratio as a variable rather than just focusing on a gonadotropin index.In this study,LH showed a downward trend compared with that before the operation,the change in FSH showed periodic changes,and LH/FSH was significantly improved compared with that before the operation.During the followup,it was also found that the menstrual cycle of 27 patients returned to normal,and 6 patients with pregnancy intention(18%) became pregnant within 1 year after the operation.According to the guidelines,women of childbearing age should avoid pregnancy for at least one year after weight loss,mainly because of the patient's own nutritional status and physical recovery.In this study,some patients became pregnant within 1 year after the operation due to age,family and other factors;that is,laparoscopic sleeve gastrectomy has a definite effect on obesity complicated with polycystic ovary syndrome.

The fasting insulin level reflects compensatory hyperinsulinemia.The diagnostic criterion of insulin resistance is that fasting insulin is greater than or equal to 85 pmol/L,especially in patients with hypertension and obesity.Fat accumulation often weakens the biological activity of insulin and makes the body resistant to insulin.Although the effects of obesity are different,it interferes with the pathophysiology of PCOS and affects insulin resistance and hyperinsulinemia[15].Hyperinsulinemia stimulates androgen production and maintains abnormal gonadotropin secretion in the pituitary gland.In contrast,weight loss can improve insulin resistance,reduce circulating LH concentration,and increase reproductive potential[18].In this study,it was observed that the level of serum insulin decreased from 34.2 mIU/mL to 9 mIU/mL,which reflected the improvement of insulin resistance and had a positive effect on the attenuation of androgen,resulting in the secretion of gonadotropin tending to normal.The above results can be explained by the increase in postoperative gastric emptying,which further leads to a decrease in GLP-1-mediated insulin secretion and related auxinreleasing peptide and leptin,thus increasing serum insulin and reducing insulin resistance[19].

In this study,EWL% finally reached 65% and stabilized 12 mo after the operation,and BMI gradually decreased to 28.98 at 12 mo after the operation (P＜ 0.05),which confirmed the significant weight loss effect of LSG.During the postoperative follow-up,it was found that 15 patients with acanthosis nigricans improved to varying degrees.At present,the etiology of acanthosis nigricans is not clear,and it is often associated with abnormal metabolism of hormones and insulin and is often associated with polycystic ovary syndrome.It is undeniable that the improvement of acanthosis nigricans is related to polycystic ovary syndrome.However,the specific mechanism and causality still need to be further studied.Hyperandrogenemia can also show hirsutism,especially in female patients.Although there was no specific score in this study,it was found that the endocrine and metabolic indices gradually stabilized and hirsutism significantly improved during the follow-up.

CONCLUSION

In summary,LSG can improve hyperandrogenaemia and irregular menstruation in obese patients with PCOS,significantly reduce weight loss and improve a series of complications related to PCOS.such as acanthosis nigricans and hirsutism.Laparoscopic sleeve gastrectomy can improve patients' physical,hormonal and reproductive indicators and can be considered part of the treatment of infertility in obese patients.Further research is needed to draw more reliable conclusions.

ARTICLE HIGHLIGHTS

Research background

Polycystic ovary syndrome (PCOS) is a common endocrine disease in young women,with a prevalence rate of 5%-18%.PCOS is closely related to obesity,and an increase in obesity will enhance the severity and expression of the PCOS phenotype.

Research motivation

The efficacy of laparoscopic sleeve gastrectomy (LSG) for obesity with PCOS are still unclear.

Research objectives

The purpose of the study was to investigate the effect of LSG on related variables in obese patients with PCOS.

Research methods

The clinical data of 32 patients who were diagnosed with obesity complicated with polycystic ovary syndrome and underwent laparoscopic sleeve gastrectomy from January 2013 to December 2020 were reviewed.The changes in anthropometric indices,insulin,testosterone,estradiol,follicle stimulating hormone (FSH),luteinizing hormone (LH),menstrual cycle and LH/FSH ratio before and 1 mo,3 mo,6 mo and 12 mo after the operation were statistically analyzed.

Research results

The patient's body mass and BMI were basically stable at 1 year after the operation.During the postoperative period,the average level of estradiol increased;the average serum testosterone and the insulin level decreased significantly after the operation.On the other hand,after surgical treatment,it was found that the average ratio was less than 1,indicating that the average FSH level after bariatric surgery was higher than that of LH.

Research conclusions

Laparoscopic sleeve gastrectomy can improve hyperandrogenaemia and irregular menstruation in obese patients with polycystic ovary syndrome,significantly reduce weight loss and improve a series of complications related to polycystic ovary syndrome.

Research perspectives

The impact of laparoscopic sleeve gastrectomy on obese patients with polycystic ovary syndrome's physical,hormonal and reproductive indicators.

FOOTNOTES

Author contributions:Wang XT and Han JL contributed to protocol/project development;Wang XT,Hou YS,Zhao HL,Wang J,Guo CH,and Han JL contributed to data collection or management;Wang XT,Guan J,Lv ZG,Ma P,and Han JL contributed to data analysis;Wang XT,and Han JL contributed to manuscript writing/editing;All authors reviewed the manuscript.

Supported byShanxi Province “136” Revitalization Medical Project Construction Funds,No.2019XY003.

Institutional review board statement:The study was reviewed and approved by the Shanxi Bethune Hospital,Shanxi Academy of Medical Sciences Review Board.

Informed consent statement:All study participants or their legal guardian provided informed written consent about personal and medical data collection prior to study enrollment.

Conflict-of-interest statement:All the authors report no relevant conflicts of interest for this article.

Data sharing statement:No additional data are available.

Open-Access:This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers.It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license,which permits others to distribute,remix,adapt,build upon this work non-commercially,and license their derivative works on different terms,provided the original work is properly cited and the use is non-commercial.See: https://creativecommons.org/Licenses/by-nc/4.0/

Country/Territory of origin:China

ORCID number:Hao-Liang Zhao 0000-0002-9136-498X;Jian-Li Han 0000-0001-7791-3519.

S-Editor:Liu JH

L-Editor:A

P-Editor:Cai YX