侧脑室内注射神经肽S对瑞芬太尼所致痛觉过敏小鼠镇痛效果的研究

丁晨 马婷婷 胡兵伟

[摘要] 目的 研究側脑室内注射神经肽S对瑞芬太尼痛觉过敏小鼠镇痛效果的影响。 方法 将雌性SPF级BALB/c小鼠随机分为对照组、切口痛组、瑞芬太尼组和切口痛-瑞芬太尼组。待切口痛-瑞芬太尼组小鼠造模成功后侧脑室注射给药，分为NS（生理盐水）组和2 nmol/L NPS组（神经肽S）、4 nmol/L NPS组、6 nmol/L NPS组、8 nmol/L NPS组。通过侧脑室注射不同剂量NPS及NS，检测小鼠机械痛缩足阈值和热辐射缩足潜伏期。 结果与同组基础值相比，术后切口痛组、瑞芬太尼组和切口痛-瑞芬太尼组的机械痛缩足阈值和热辐射缩足潜伏期明显减少;术后6 h、24 h、48 h与对照组相比，切口痛组、瑞芬太尼组和切口痛-瑞芬太尼组的机械痛缩足阈值和热辐射缩足潜伏期明显减少;成功构建切口痛-瑞芬太尼组痛觉过敏小鼠模型并侧脑室注射给药后，与NS组相比较，NPS组给药后的小鼠机械痛缩足阈值和热辐射缩足潜伏期明显增加;与给药前相比，NPS各浓度组的小鼠机械痛缩足阈值和热辐射缩足潜伏期在给药后30 min内随着时间的延长而增加。 结论 在切口痛-瑞芬太尼痛觉过敏小鼠疼痛模型中，侧脑室注射NPS对瑞芬太尼引起的痛觉过敏有明显的镇痛效果。

[关键词] 神经肽S;瑞芬太尼;镇痛效果;痛觉过敏

[中图分类号] R614          [文献标识码] A          [文章编号] 1673-9701（2020）12-0037-05

[Abstract] Objective To study the analgesic effect of intraventricular injection of neuropeptide S on the analgesic effect of remifentanil-induced hyperalgesia in mice. Methods Female SPF BALB/c mice were randomly divided into control group， incision pain group， remifentanil group and incision pain-remifentanil group. After the incisional pain-remifentanil-induced hyperalgesia mice were successfully modelled， the drugs were injected into lateral ventricle and the mice were divided into the NS （normal saline） group and the 2 nmol/L NPS group （neuropeptide S）， the 4 nmol/L NPS group， 6 nmol/L NPS group， 8 nmol/L NPS group. By injecting different doses of NPS and NS into the lateral ventricle， the threshold of mechanical pain withdrawal and the latency of thermal radiation withdrawal were measured. Results Compared with the basic values in the same group， the postoperative incision pain group， the remifentanil group and the incision pain-remifentanil group had significantly reduced mechanical foot withdrawal thresholds and thermal radiation foot withdrawal latency. Compared with the control group at 6 h， 24 h and 48 h after operation， the incision pain group， remifentanil group， and incision pain-remifentanil group had significantly reduced mechanical foot withdrawal thresholds and thermal radiation foot withdrawal latency. After the model of hyperalgesia in the incision pain remifentanil group was successfully constructed and injected into the lateral ventricle， compared with NS group， the mechanical pain foot retraction threshold and thermal radiation foot retraction latency in NPS group increased significantly. Compared with those before administration， the mechanical pain withdrawal threshold and thermal radiation withdrawal latency of mice in each concentration group of NPS increased with time within 30 min after administration. Conclusion In mice model of incisional pain-remifentanil-induced hyperalgesia， lateral ventricle injection of NPS has a significant analgesic effect on remifentanil-induced hyperalgesia.