Stability study of resveratrol-loaded emulsions using pectin as an emulsifer

aDepartment of Pharmaceutical Technology,Silpakorn University,Nakhon Pathom 73000,Thailand

bPharmaceutical Biopolymer Group(PBiG),Faculty of Pharmacy,Silpakorn University,Nakhon Pathom 73000, Thailand

Stability study of resveratrol-loaded emulsions using pectin as an emulsifer

Porntip Benjasirimongkola,b,*,Pornsak Sriamornsaka,b

aDepartment of Pharmaceutical Technology,Silpakorn University,Nakhon Pathom 73000,Thailand

bPharmaceutical Biopolymer Group(PBiG),Faculty of Pharmacy,Silpakorn University,Nakhon Pathom 73000, Thailand

A R T I C L E I N F O

Article history:

Available online 25 November 2015

Resveratrol

Pectin

Emulsion stability

Homogenization

Phenolic compound such as resveratrol has been suggested to prevent cancer and heart diseases because of its anti-oxidant and anti-infammatory activities[1].However,therapeutic application of resveratrol is quite limited due to its poor solubility, low bioavailability,photosensitive and auto-oxidation properties[2].The aim of this study was to prepare resveratrolloaded emulsions using pectin as an emulsifer.The emulsions should be stable for up to 7 days,before further manufacturing processes.

Resveratrol-loaded emulsions were prepared by simple homogenization using Imwitor®742 as an oil phase(10%w/w). Resveratrol was dissolved in Imwitor®742;solubility of resveratrol in Imwitor®742 was 9.53±0.52 mg/mL.The infuence of type(i.e.,low methoxy pectin(LMP)and high methoxy pectin(HMP))and concentration(i.e.,2%and 3%w/w)of pectin on the droplet size,zeta potential,viscosity,stability(by visually observed percent creaming and automatic stability analysis) and morphology of emulsions were examined.The stability of emulsions after storage at ambient temperature(25°C)for 7 days and under environmental stress condition(i.e., centrifugation test)was compared with the freshly prepared emulsions(day 0).The microscopic image of emulsions containing HMP(3%w/w)and resveratrol is shown in Fig.1A.The emulsion droplet size was increased from 19.34±2.6 μm(day 0)to 26.95±1.76 μm(day 7).From visual observation,emulsions containing HMP(3%w/w)and resveratrol did not show phase separation(100%creaming)after centrifugation test and 7-day stability test.However,the results by automatic stability analyzer showed the difference between the emulsions after centrifugation test and 7-day stability test(Fig.1B).It could be concluded that the emulsions containing HMP(3%w/w)and resveratrol provide the most stable emulsions compared to other formulations.

Acknowledgment

The authors wish to acknowledge Herbstreith&Fox KG (Germany)who kindly donate pectin samples.

Fig.1–(A)Microscopic image and(B)backscattering patterns of emulsions containing HMP(3%w/w)and resveratrol.

R E F E R E N C E S

[1]Pace-Asciak CR,Hahn S,Diamandis EP,et al.The red wine phenolics trans-resveratrol and quercetin block human platelet aggregation and eicosanoid synthesis:implications for protection against coronary heart disease.Clin Chim Acta 1995;235:207–219.

[2]Amri A,Chaumeil JC,Sfar S,et al.Administration of resveratrol:what formulation solutions to bioavailability limitations?J Control Release 2012;158:182–193.

*E-mail address:benjasirimongko_p@silpakorn.edu.

Peer review under responsibility of Shenyang Pharmaceutical University.

http://dx.doi.org/10.1016/j.ajps.2015.11.037

1818-0876/©2016 Production and hosting by Elsevier B.V.on behalf of Shenyang Pharmaceutical University.This is an open access article under the CC BY-NC-ND license(http://creativecommons.org/licenses/by-nc-nd/4.0/).