Formulation development and preparation of fsh oil liposome by using high pressure homogenizer for food supplement product

Thanaporn Amnuaikit

Faculty of Pharmaceutical Sciences,Prince of Songkla University,Hat Yai,Songkhla 90112,Thailand

Formulation development and preparation of fsh oil liposome by using high pressure homogenizer for food supplement product

Thanaporn Amnuaikit*

Faculty of Pharmaceutical Sciences,Prince of Songkla University,Hat Yai,Songkhla 90112,Thailand

A R T I C L E I N F O

Article history:

Available online 25 November 2015

Fish oil

Liposome

High pressure homogenizer

Food supplement product

It is known that“Fish Oil”is the raw material that has lot of benefts for health,because fsh oil consists of several necessary unsaturated fatty acids,particularly Omega-3 and Docosahexanoic acid(DHA).Omega-3 can decrease triglyceride level,and then it can increase HDL cholesterol level.In addition,DHA can support brain cell synthesis and also nervous system for human.However,fsh oil has fshy smell and uncomfortable to eat.Nanotechnology,particularly liposome is a good choice for fsh odor smell reduction and increase product acceptability[1].Furthermore,nanotechnology improved oral dose and bioavailability of the fsh oil liposome[2].

Fish oil liposomes were developed for determination of suitable ratio of liposome structure making substances that incorporated 14%w/w of fsh oil in formulations.They were frstly prepared by modifed ethanol injection using rotary evaporator[3].The physical characteristics of formulations were defned as quality of colloidal system which no separation or sedimentation within 3 months in room temperature (30±2°C)and optimal pH values.The good appearance of formulation was chosen to further prepare by using high pressure homogenizer(Fig.1A).The suitable ratio of liposome composition was developed and scaled up to 10 kilograms of fsh oil liposome preparation.Both of particles size and zeta potential of fsh oil liposome were analyzed by Zetasizer. The characteristic of particle was determined by scanning electron microscopy(SEM)as well(Fig.1C).Moreover,fsh oil liposome inspection was included pH value,microbiological test and nutrition test.Results showed that the good formulation of fsh oil liposome was composed of phosphatidylcholine from soybean,cholesterol,span 80,tween 80 and preservative.Size of vesicle was 75.6±3 nm and zeta-potential value was−44.29±6.14 mV.Particle dispersion index(PDI)was 0.267±0.007 and pH value was 4.53±0.11.There were no microbial growth of Salmonella spp.,Staphylococcus aureus,Bacillus cereus,Vibrio cholera and Vibrio parahaemolyticus.Peroxide and unsaponifable matter value were in the range of standard for edible oil or lipid.Omega-3 content was 3000 mg/ 15 ml of fsh oil liposome.It might be concluded that fsh oil liposome product which prepared by high pressurehomogenizer was shown as high quality.This technique should be recommended for fsh oil liposome processing in food supplement.

Fig.1–High pressure homogenizer(A);Fish oil liposome product(B);Liposome particles from SEM magnifed 1×106(C).

Acknowledgements

The authors acknowledge the fnancial support received from National Innovation Agency,Thailand and Kuang Pei San Food Products Public Co.,Ltd,for their fulflls and encouragement in this work.

R E F E R E N C E S

[1]Keller BC.Liposomes in nutrition.Trends Food Sci Technol 2001;12:25–31.

[2]Liu W,Liu WL,Liu CM,et al.Medium-chain fatty acid nanoliposomes for easy energy supply.Nutrition 2011;27:700–706.

[3]Pinsuwan S,Amnuaikit T,Ungphaiboon S,et al.Liposomecontaining Hibiscus sabdariffa Calyx extract formulations with increased antioxidant activity,improved dermal penetration and reduced dermal toxicity.J Med Assoc Thai 2010;93:216–226.

*E-mail address:chomchan.a@psu.ac.th.

Peer review under responsibility of Shenyang Pharmaceutical University.

http://dx.doi.org/10.1016/j.ajps.2015.11.094

1818-0876/©2016 Production and hosting by Elsevier B.V.on behalf of Shenyang Pharmaceutical University.This is an open access article under the CC BY-NC-ND license(http://creativecommons.org/licenses/by-nc-nd/4.0/).