五叶地锦果实多糖、花色苷抗氧化、抗肿瘤活性研究

柴军红　何婷婷　赵楠　金志民

摘要：采用體外抗氧化体系研究五叶地锦（Parthenocissus quinquefolia）果实多糖、花色苷对超氧自由基、羟基自由基、DPPH自由基的清除能力，采用MTT法研究果实中花色苷和多糖对两种肝癌细胞（HepG2，SMMC-7221）的抑制作用。结果表明，多糖和花色苷浓度与肝癌细胞的抑制率具有剂量依赖关系，当花色苷浓度达到0.312 5 mg/mL时，对两种肝癌细胞的抑制率分别为37.89%±1.74%、53.57%±1.72%;当多糖提取液浓度达到0.312 5 mg/mL时，对两种肝癌细胞的抑制率分别为79.59%±0.52%、58.85%±1.12%;多糖、花色苷浓度在0.80 mg/mL时对超氧自由基的清除率分别达到74.21%±2.48%、90.06%±0.86%;对羟基自由基的清除率达到75.87%±1.61%、68.60%±1.52%;对DPPH自由基的清除率达到91.41%±0.74%、90.73%±0.44%。另外，羟基自由基、超氧自由基清除能力与抗肿瘤之间显示相关性，与DPPH相关性较差。五叶地锦果实中提取的多糖和花色苷对肝癌细胞具有一定抑制作用，同时表现出好的抗氧化能力，且两者之间存在明显相关性，具有开发潜力。

关键词：五叶地锦（Parthenocissus quinquefolia）果实;花色苷;多糖;抗氧化;MTT

中图分类号：R965.1;R735.7         文献标识码：A

文章编号：0439-8114（2019）15-0097-05

DOI：10.14088/j.cnki.issn0439-8114.2019.15.023           开放科学（资源服务）标识码（OSID）：

Study on antioxidant and antitumor activity of polysaccharide

and anthocyanin from Parthenocissus quinquefolia fruit

CHAI Jun-hong，HE Ting-ting，ZHAO Nan，JIN Zhi-min

（Mudanjiang Normal University，Mudanjiang 157011，Heilongjiang，China）

Abstract： In vitro the antioxidant activity of polysaccharide and anthocyanin on Parthenocissus quinquefolia fruit was discussed. Study on the scavenging ability of polysaccharide and anthocyanin with superoxide free radical， hydroxyl radical and DPPH free radical. Using MTT method to study the anthocyanins and polysaccharides in inhibition of 2 kinds of hepatoma cells（HepG2， SMMC-7221）. The results showed that the polysaccharide and anthocyanin concentration has a dose-dependent relationship with the HCC inhibitory rate， when the anthocyanin concentration reached 0.312 5 mg/mL， the inhibition rate of two HCC was 37.89%±1.74%， 53.57%±1.72%; when the polysaccharide concentration reached 0.312 5 mg/mL， the inhibition rate of two HCC cells was 79.59%±0.52%， 58.85%±1.12%. The polysaccharide or anthocyanin was 0.80 mg/mL， the scavenging rate of superoxide radicals was 74.21%±2.48%、90.06%±0.86%， the hydroxyl radical was 75.87%±1.61%、68.60%±1.52%， the scavenging rate of DPPH was 91.41%±0.74%、90.73%±0.44%. Furthermore，the scavenging ability of hydroxyl radical has a correlation with antitumor. The superoxide radicals also showed certain correlation， and the correlation of DPPH was poor. Parthenocissus quinquefolia fruit polysaccharide and anthocyanin has certain inhibitory effect on HCC and radical， there is a clear correlation with antioxidant and antitumor;it has the development potential.