AUTHOR INSTRUCTIONS

1. Submission Overview

Before you decide to publish with us, please read the following items carefully and make sure that you are well aware of our policies and requirements.

1.1 Topic Suitability

The topic of the manuscript mustfit the scope of the journal. Please refer to Aims and Scope for more information.

1.2 Open Access and Copyright

The journal adopts Gold Open Access publishing model and distributes content under the Creative Commons Attribution 4.0 International License. Please make sure that you are well aware of these policies.

1.3 Publication Fees

Authors are required to pay Article Processing Charges of 299 US Dollars after the manuscript is officially accepted. For more details, please refer to Article Processing Charges.

1.4 Language Editing

All submissions are required to be presented clearly and cohesively in good English. Authors whosefirst language is not English are advised to have their manuscripts checked or edited by a native English speaker before submission to ensure the high quality of expression. A well-organized manuscript in good English would make the peer review even the whole editorial handling more smooth and efficient.

If needed, authors are recommended to consider the language editing services provided by Charlesworth to ensure that the manuscript is written in correct scientific English before submission. Authors who publish with OAE journals enjoy a special discount for the services of Charlesworth.

Additional data and information can be uploaded as Supplementary Material to accompany the manuscripts. The supplementary materials will also be available to the referees as part of the peer-review process. Anyfile format is acceptable, such as data sheet (word, excel, csv, cdx, fasta, pdf or zipfiles), presentation (powerpoint, pdf or zipfiles), image(cdx, eps, jpeg, pdf, png or tiff), table (word, excel, csv or pdf), audio (mp3, wav or wma) or video (avi, divx, flv, mov, mp4,mpeg, mpg or wmv). All information should be clearly presented. Supplementary materials should be cited in the main text in numeric order (e.g., Supplementary Figure 1, Supplementary Figure 2, Supplementary Table 1, Supplementary Table 2,etc.). The style of supplementaryfigures or tables complies with the same requirements onfigures or tables in main text.Videos and audios should be prepared in English, and limited to a size of 500 MB or a duration of 3 minutes.

1.5 Work Funded by the National Institutes of Health

2.3.3.9 References

2. Submission Preparation

2.1 Cover Letter

A cover letter is required to be submitted accompanying each manuscript. It should be concise and explain why the study is significant, why itfits the scope of the journal, and why it would be attractive to readers,etc.

2.3.3.7 Consent for Publication

In thefirst paragraph: include the title and type (e.g., Original Article, Review, Case Report,etc.) of the manuscript, a brief on the background of the study, the question the author sought out to answer and why;

In the second paragraph: concisely explain what was done, the mainfindings and why they are significant;

In the third paragraph: indicate why the manuscriptfits the Aims and Scope of the journal, and why it would be attractive to readers;

In the fourth paragraph: confirm that the manuscript has not been published elsewhere and not under consideration of any other journal. All authors have approved the manuscript and agreed on its submission to the journal. Journal’s specific requirements have been met if any.

If the manuscript is contributed to a special issue, please also mention it in the cover letter.

羽化后2～10日龄的斯氏按蚊呈现较高的吸血活性，此时已受精的雌蚊急需足量的血液维持卵泡发育、产卵等生命活动；但随着蚊龄的增加，雌蚊卵巢内非功能性变形卵泡数量不断增加，雌蚊的受精能力也随之下降。因此，按蚊的吸血活性也逐渐降低。以上结果表明，蚊虫的吸血行为受自身的生命规律的影响，比如生命周期性、昼夜节律性、叮刺周期特性、室内/室外吸血偏好性等［11-12］。

If the manuscript was presented partly or entirely in a conference, the author should clearly state the background information of the event, including the conference name, time and place in the cover letter.

主要分布在研究区东部的青衣江两侧，涉及峨眉山市和夹江县，另外在马边县县城一带见一平坝。3块平原(坝)面积494.98 km2。

2.2 Types of Manuscripts

There is no restriction on the length of manuscripts, number offigures, tables and references, provided that the manuscript is concise and comprehensive. The journal publishes Original Article, Review, Meta-analysis, Case Report, Commentary,etc.For more details about paper type, please refer to the following table.

2.3 Manuscript Structure

2.3.1 Front Matter

2.3.1.1 Title

The title of the manuscript should be concise, specific and relevant, with no more than 16 words if possible. When gene or protein names are included, the abbreviated name rather than full name should be used.

采用SPSS 20.0统计学软件对数据进行处理，计数资料以百分数（%）表示，采用x2检验；计量资料以“±s”表示，采用t检验，以P＜0.05为差异有统计学意义。

以SPSS11.0软件进行数据处理。对计量资料行正态分布及方差齐性检验，以（± s）表示，两组均数间比较采用t检验；计数资料用百分率表示，采用χ2检验；以P＜0.05，差异有统计学意义。

2.3.1.2 Authors and Affiliations

Authors’ full names must be listed. The initials of middle names can be provided. Institutional addresses and email addresses for all authors should be listed. At least one author should be designated as corresponding author. In addition,corresponding authors are suggested to provide their Open Researcher and Contributor ID upon submission. Please note that any change to authorship is not allowed after manuscript acceptance.

2.3.1.3 Abstract

The abstract should be a single paragraph with word limitation and specific structure requirements (for more details please refer to Types of Manuscripts). It usually describes the main objective(s) of the study, explains how the study was done,including any model organisms used, without methodological detail, and summarizes the most important results and their significance. The abstract must be an objective representation of the study: it is not allowed to contain results which are not presented and substantiated in the manuscript, or exaggerate the main conclusions. Citations should not be included in the abstract.

2.3.1.4 Keywords

Three to eight keywords should be provided, which are specific to the article, yet reasonably common within the subject discipline.

同时使用两台摊铺机成梯队进行单幅一次性铺筑，摊铺宽度分别为5.5m与5.0m，摊铺机料位难以到边位置，应

2.3.2 Main Text

Manuscripts of different types are structured with different sections of content. Please refer to Types of Manuscripts to make sure which sections should be included in the manuscripts.

2.3.2.1 Introduction

The introduction should contain background that puts the manuscript into context, allow readers to understand why the study is important, include a brief review of key literature, and conclude with a brief statement of the overall aim of the work and a comment about whether that aim was achieved. Relevant controversies or disagreements in thefield should be introduced as well.

2.3.2.2 Methods

Methods should contain sufficient details to allow others to fully replicate the study. New methods and protocols should be described in detail while well-established methods can be brie fly described or appropriately cited. Experimental participants selected, the drugs and chemicals used, the statistical methods taken, and the computer software used should be identified precisely. Statistical terms, abbreviations, and all symbols used should be defined clearly. Protocol documents for clinical trials, observational studies, and other non-laboratory investigations may be uploaded as supplementary materials.

2.3.2.3 Results

This section contains thefindings of the study. Results of statistical analysis should also be included either as text or as tables orfigures if appropriate. Authors should emphasize and summarize only the most important observations. Data on all primary and secondary outcomes identified in the section Methods should also be provided. Extra or supplementary materials and technical details can be placed in supplementary documents.

2.3.2.4 Discussion

This section should discuss the implications of thefindings in context of existing research and highlight limitations of the study. Future research directions may also be mentioned.

2.3.2.5 Conclusion

It should state clearly the main conclusions and include the explanation of their relevance or importance to thefield.

A brief overview of the video or audiofiles should be given in the manuscript text.

2.3.3.1 Acknowledgments

Anyone who contributed towards the article but does not meet the criteria for authorship, including those who provided professional writing services or materials, should be acknowledged. Authors should obtain permission to acknowledge from all those mentioned in the Acknowledgments section. This section is not added if the author does not have anyone to acknowledge.

2.3.3.2 Authors’ Contributions

Each author is expected to have made substantial contributions to the conception or design of the work, or the acquisition,analysis, or interpretation of data, or the creation of new software used in the work, or have drafted the work or substantively revised it.

Please use Surname and Initial of Forename to refer to an author’s contribution. For example: made substantial contributions to conception and design of the study and performed data analysis and interpretation: Salas H, Castaneda WV; performed data acquisition, as well as provided administrative, technical, and material support: Castillo N, Young V.

If an article is single-authored, please include “The author contributed solely to the article.” in this section.

在确定施工工艺后，需要对施工过程中的温度加以严格控制：①将沥青加热温度控制在150～160℃范围内；②将集料加热温度控制在160～180℃范围内；③将混合料的出厂温度控制在155～165℃范围内；④摊铺时，混合料温度应达到135℃以上；⑤碾压时，混合料温度应达到130℃以上；⑥碾压终了时，混合料温度应达到80℃以上。

2.3.3.3 Availability of Data and Materials

In order to maintain the integrity, transparency and reproducibility of research records, authors should include this section in their manuscripts, detailing where the data supporting theirfindings can be found. Data can be deposited into data repositories or published as supplementary information in the journal. Authors who cannot share their data should state that the data will not be shared and explain it. If a manuscript does not involve such issue, please state “Not applicable.” in this section.

2.3.3.4 Financial Support and Sponsorship

All sources of funding for the study reported should be declared. The role of the funding body in the experiment design,collection, analysis and interpretation of data, and writing of the manuscript should be declared. Any relevant grant numbers and the link of funder’s website should be provided if any. If the study is not involved with this issue, state “None.” in this section.

2.2.5 不同月份和处理对载畜量影响的双因素方差分析 通过月和处理对载畜量影响的双因素方差分析可知(表7)，月、处理以及二者之间的互作效应均对载畜量有极显著的影响(P<0.01)，其中月对载畜量的影响最显著。处理对DCP载畜量的影响最大(F=169.98)，对DM载畜量的影响次之(F=111.54)，对ME载畜量的影响极小(F=9.97)。

2.3.3.5 Con flicts of Interest

Authors must declare any potential con flicts of interest that may be perceived as inappropriately in fluencing the representation or interpretation of reported research results. If there are no con flicts of interest, please state “All authors declared that there are no con flicts of interest.” in this section. Some authors may be bound by confidentiality agreements.In such cases, in place of itemized disclosures, we will require authors to state “All authors declare that they are bound by confidentiality agreements that prevent them from disclosing their con flicts of interest in this work.”. If authors are unsure whether con flicts of interest exist, please refer to the “Con flicts of Interest” of OAE Editorial Policies for a full explanation.

2.3.3.6 Ethical Approval and Consent to Participate

Research involving human subjects, human material or human data must be performed in accordance with the Declaration of Helsinki and approved by an appropriate ethics committee. An informed consent to participate in the study should also be obtained from participants, or their parents or legal guardians for children under 16. A statement detailing the name of the ethics committee (including the reference number where appropriate) and the informed consent obtained must appear in the manuscripts reporting such research.

Studies involving animals and cell lines must include a statement on ethical approval. More information is available at Editorial Policies.

那么上述合理性的论证是否同样适用于那些被权利用尽区分适用理论所排除的产品呢？在这种区别适用理论中，“专门用于执行专利方法的产品”、“专门用于制造专利商品的零部件或设备”被排除出了权利用尽规则的范围。

If the manuscript does not involve such issue, please state “Not applicable.” in this section.

Here is a guideline of a cover letter for authors’ consideration:

Manuscripts containing individual details, images or videos, must obtain consent for publication from that person, or in the case of children, their parents or legal guardians. If the person has died, consent for publication must be obtained from the next of kin of the participant. Manuscripts must include a statement that a written informed consent for publication was obtained. Authors do not have to submit such content accompanying the manuscript. However, these documents must be available if requested. If the manuscript does not involve this issue, state “Not applicable.” in this section.

2.3.3.8 Copyright

Authors retain copyright of their works through a Creative Commons Attribution 4.0 International License that clearly states how readers can copy, distribute, and use their attributed research, free of charge. A declaration “© The Author(s)2018.” will be added to each article. Authors are required to sign License to Publish before formal publication.

If an accepted manuscript was funded by National Institutes of Health (NIH), the author may inform editors of the NIH funding number. The editors are able to deposit the paper to the NIH Manuscript Submission System on behalf of the author.

References should be numbered in order of appearance at the end of manuscripts. In the text, reference numbers should be placed in square brackets and the corresponding references are cited thereafter. All authors’ names are required to be listed in the references. Abbreviations of the journals should be provided on the basis of Index Medicus. Information from manuscripts accepted but not published should be cited in the text as “Unpublished material” with written permission from the source.

对所有长度-直径矩阵中的模型都进行了模拟，并且选取了一些具有代表性的结果进行对比。首先展示出来的是长度固定，然后改变树根直径。

References should be described as follows, depending on the types of works:

小学道德与法治课堂教学进行教法升级符合新课改倡导的基本精神。小学身心成长有自身规律，教师要巧妙借助教材中的插图，点燃学生学习热情；善于运用生活中的故事，拓展学生思维认知；组织趣味游戏，内化学生道德情感；探析典型案例，升级学生法制意识。通过这些方式，课堂教学实现了跨越式发展，学生良好品德自然生成。

For other types of references, please refer to U.S. National Library of Medicine.

The journal also recommends that authors prepare references with a bibliography software package, such as EndNote to avoid typing mistakes and duplicated references.

2.3.3.10 Supplementary Materials

More details are available at http://www.charlesworthauthorservices.com/.

实训课程开课前，由教研组教师集中讨论，选择出需要与患者互动的基护操作，设计适用该操作的病例，并针对病例设计对话。对话要求简洁明了，能够体现该项操作特点与注意事项，符合临床实际。在上课前将病例和对话交给护生，要求其熟悉病例，熟练掌握对话内容。实训课上，先由教师示教，强调对话内容，然后督促护生进行小组练习，结合提前掌握的对话内容，分角色演示案例。

3、企业文化进走廊。在营销部办公室走廊里，悬挂着一幅幅艺术作品，有数字油画、摄影作品、国画作品等，这些都是出自营销部员工和员工家属之手的原创作品，使中心的环境显得更温馨，在工作之余观赏着这些作品，给人一种艺术的享受。

Agilent 1260型高效液相色谱仪，DAD检测器，美国Agilent公司；DF-102S型集热式恒温加热磁力搅拌器，河南巩义市予华有限公司；THZ-92A型恒温振荡器，翠柳市实验仪器有限公司；XW-80A型漩涡混合仪，上海医科大学仪器厂；MD400-2型氮气吹扫仪，浙江杭州奥威仪器有限责任公司；D8 Advance型X射线粉末衍射仪，瑞士Bruker公司。

2.4 Manuscript Format

2.4.1 File Format

Manuscriptfiles can be in DOC and DOCX formats and should not be locked or protected.

2.4.2 Length

There are no restrictions on paper length, number offigures, or amount of supporting documents. Authors are encouraged to present and discuss theirfindings concisely.

2.4.3 Language

Manuscripts must be written in English.

2.4.4 Multimedia Files

The journal supports manuscripts with multimediafiles. The requirements are listed as follows:

Videos or audiofiles are only acceptable in English. The presentation and introduction should be easy to understand. The frames should be clear, and the speech speed should be moderate.

2.3.3 Back Matter

The video or audiofiles should be limited to a duration of 3 min and a size of up to 500 MB.

Please use professional software to produce high-quality videofiles, to facilitate acceptance and publication along with the submitted article. Upload the videos in mp4, wmv, or rm format (preferably mp4) and audiofiles in mp3 or wav format.

2.4.5 Figures

Figures should be cited in numeric order (e.g., Figure 1, Figure 2) and placed after the paragraph where it isfirst cited;Figures can be submitted in format of tiff, psd, AI or jpeg, with resolution of 300-600 dpi;Figure caption is placed under the Figure;Diagrams with describing words (including, flow chart, coordinate diagram, bar chart, line chart, and scatter diagram, etc.)should be editable in word, excel or powerpoint format. Non-English information should be avoided;Labels, numbers, letters, arrows, and symbols infigure should be clear, of uniform size, and contrast with the background;Symbols, arrows, numbers, or letters used to identify parts of the illustrations must be identified and explained in the legend;Internal scale (magnification) should be explained and the staining method in photomicrographs should be identified;All non-standard abbreviations should be explained in the legend;Permission for use of copyrighted materials from other sources, including re-published, adapted, modified, or partialfigures and images from the internet, must be obtained. It is authors’ responsibility to acquire the licenses, to follow any citation instruction requested by third-party rights holders, and cover any supplementary charges.

2.4.6 Tables

Tables should be cited in numeric order and placed after the paragraph where it isfirst cited;The table caption should be placed above the table and labeled sequentially (e.g., Table 1, Table 2);Tables should be provided in editable form like DOC or DOCX format (picture is not allowed);Abbreviations and symbols used in table should be explained in footnote;Explanatory matter should also be placed in footnotes;Permission for use of copyrighted materials from other sources, including re-published, adapted, modified, or partial tables from the internet, must be obtained. It is authors’ responsibility to acquire the licenses, to follow any citation instruction requested by third-party rights holders, and cover any supplementary charges.

2.4.7 Abbreviations

Abbreviations should be defined uponfirst appearance in the abstract, main text, and infigure or table captions and used consistently thereafter. Non-standard abbreviations are not allowed unless they appear at least three times in the text.Commonly-used abbreviations, such as DNA, RNA, ATP,etc., can be used directly without definition. Abbreviations in titles and keywords should be avoided, except for the ones which are widely used.

2.4.8 Italics

General italic words likevs.,et al.,etc.,in vivo,in vitro;ttest,Ftest,Utest; related coefficient asr, sample number asn,and probability asP; names of genes; names of bacteria and biology species in Latin.

2.4.9 Units

SI Units should be used. Imperial, US customary and other units should be converted to SI units whenever possible. There is a space between the number and the unit (i.e., 23 mL). Hour, minute, second should be written as h, min, s.

2.4.10 Numbers

Numbers appearing at the beginning of sentences should be expressed in English. When there are two or more numbers in a paragraph, they should be expressed as Arabic numerals; when there is only one number in a paragraph, number ＜ 10 should be expressed in English and number ＞ 10 should be expressed as Arabic numerals. 12345678 should be written as 12,345,678.

2.4.11 Equations

Equations should be editable and not appear in a picture format. Authors are advised to use either the Microsoft Equation Editor or the MathType for display and inline equations.