Literature on the law of Miao medicine in treating liver cancer

Huili Shui,Zhu Yang,Fengxi Long,Wenqi Huang,Bing Yang,Zhen Zhang,Dongxin Tang,2*

1GuiyangCollege of Traditional ChineseMedicine,Guizhou,Guiyang,550002;2Tate administration of traditionalChinese medicinekey laboratorymiaomedical Miaomedicine forthetreatmentofchronic pain,Guizhou,Guiyang,550002.

Introduction

Livercanceris one of thecommon malignant tumorsofthedigestivetract,rankingfourth in the incidence rate in China[1].It has hidden onset,highdegree of malignancy,poor prognosis,and difficult treatment,and is knownas the"king of cancer".Miao medicineis a branch oftraditional medicine in China,with distinctive national characteristics.It hasa long history and a wide variety of drug resources,which are of high researchanddevelopment value and worthfurther explorationsince ancient times.Inorder to find the drug law of Miao medicaltreatmentoflivercancer,thisstudy collectedand analyzedthe literature relatedtoMiao medical treatment of liver cancer,in order toenrichtheliteraturefoundation of Miao medicaltreatmentof liver cancer and promote the development of Miao medicine.

Materials and methods

Data source

Inthis study,the drugnamewas derived from the book"Chinese materiamedica[2]"compiledbythe state administration of traditional Chinese medicine.

Search method

"Drugname"and "tumor","drug name"and"cancer"were searchedforthe firsttime,and then"liver cancer"or"liver tumor"weresearched for the second time in the retrieved literature.

Inclusioncriteria

Clinical literature on thetreatmentofliver cancer with concomitant single - taste vaccine;Pharmacologicalexperimental literatures onthe treatment of hepatocellularcarcinomawithsingleherb medicine of guilder;Theresearch literature about thereasonableplanand clearcurative effect of the experiment in gansu.

Exclusion criteria

Summaryandliteratureof experienceintroduction;Only onepaper wascollected fromtheliterature of thesame researchcontent.Chemotherapy isinthe literature in combination.

Research methods Specification for name of seedling and medicine

Accordingto"Chinese materiamedica&seedling medicine volume",the name oftheseedling medicine was uniformly standardized as an academicname.If shaostrange,busabig,nest long bi youngcollectivelyreferred toasa cloud;Biganza,Gabon,the realpartyiscollectively referred to as August melon;Swollen wind,beans you Europe horn,bone tea collectively known as nine tea.

Standard ofseedling medicineThe seedling medicine theory divides the seedling medicine into two kinds:cold and hot.As the medicine recorded in"Chinese materia medica seedling medicine volume"has the property of cold,cold,cool,hot,slightly cold,slightly cold,slightly cold and slightly hot,it is standardized to treat it.Cold,cool,cold,slightly cold,slightly cold and slightly cold are unified as cold.Heat and microheat are unified as heat.

Specificationof drugtasteof seedlingmedicine

According to thetheory of Miaomedicine,the Miao medicine isdivided into eighttastes:bitter,hot,sweet,astringent,sour,flax,salty and weak.Due to the influence oftraditionalChinese medicine(TCM)on"Chinesemateria medica",basedon the aboveeightdrugs,thereare also two flavorsofgan andxin.To standardizeit,xinand wei-xin medicinewillbe unified intohot taste,gan,wei-gan medicineinto sweettaste.The total numberofdrugflavors was takenasthe statistical analysis objectbecause some oftheseedling drugs had various drug flavors.

The regulationofseedling medicineThe seedling medicine theorydividestheseedling medicineinto five meridians:hot longitude,coldlongitude,fast longitude,slow longitude and halflongitude.For example,sevenleaflotus,octagon,anacorn incense,suchas the effect ofventilationandpain,blood dispersingdetumescence,usedinthetreatmentof thebodyof livingcreatures can be weak or external coldevil attackdrugs,classified as cold meridian;Sanzhishan,flying dragon's palm blood,jiujie tea andother functions such asclearing heatand cooling blood,purgingfireanddetoxifying,are used totreat thebody oflivingcreaturescan be hyperactiveoroutside thetoxicinvasion of drugs,which are classified asthermalclassics;The effect oflobelia,polygonummultiflorum and wormseed wormseedwas fast,andit was used totreat sudden onsetofdiseases,andthedrugwas transferred rapidly.Thedrugeffects suchasradix aconiti,trifoliumtrifolium,and cornstarchwerealleviated andsustained,whichwere usedtotreat chronic diseases and weaksyndromes.Wormseed mustard,toad,to leaflotus,such asthetreatment of halfbody numbness, standing and walking inconveniencesuch as drugs,tobeclassified as half of the menstrual.Becausesomeofthe seedling medicineisnotasingle,thetotalnumber of the statistical object.

Specifications ofseedling and drug efficacy

According to the classificationstandards of drug efficacyof"miao pharmacology"[3],themiao drugsweredivided into six categories:anti-drug,complement,ventilation and blood dispersing drugs,anti-injury drugs,stomach-strengthening and anticirculation drugs,and other six categories.For example,kiwifruit,toad,realgar and so on drive body poison evil class medicine to call cure poison;The complements of eucommia ulmoides,asparagus officinalis,and dendrobium were used to treat weak syndromes.The drugs such as radix aconiti,carborundum,tea oil,etc.An arrow,nine parts of tea and other treatment of various types of traumatic drugs known as the treatment of injury drugs;Poria cocos,jujube,and other drugs that have a healthy spleen and stomach and regulate body functions are called as jianwei and acyclic drugs.Other drugs that are not easy to classify,such as relieving cough,reducing phlegm and relieving asthma,and calming one's mind.

The dataprocessing

Microsoft Excel2016 software was used to sort and input the collected vaccines,establish a database of drugs for treating liver cancer in the Miao medicine,and then conduct frequency and frequency statistics on their family items,drug properties,drug flavor items,regression items and efficacy items.

Results

Results ofmedicationforlivercancer in miao nationality

As is shown in table 1.After repeated review and screening,132 taste of seedling medicine was finally met.Most of them were derived from plant medicine of 119 tastes,accounting for 90.2%.Animal and mineral drugs were relatively few,accounting for 9.8%.

Table 1 Drug records of Miao medicinein treating liver cancer

?

Medicaltreatmentof livercancer ofMiao medicine belongs to the departmentofstatistics

As is shown in figure 1.A total of 30 times,accounting for 22.8 percent of the total frequency,were recorded in thehigh frequency family:compositae,liliaceae,and rosaceae.

Drugpropertystatisticsfortreatment ofliver cancerinMiaomedicine

As isshown in figure 2.The drugproperty of132 tasteof livercancer treated by Miaomedicinewas classified,amongwhichcoldmedicinewas more than100taste,accountingfor 75.8%of thetotal frequency.

Pharmacology statistics ofdrugs for treating livercancer in miao nationality

As is shown in figure 3.A total of 199 times were conducted for the treatment of 132 flavors of liver cancer in Miao medicine.Thetopthreedrugs were bitter,spicyandsweet,accountingfor 75.5%of the total frequency.

Medical treatmentofliver cancerinthe Miao medicine:statistical analysis.

As shownin figure 4.Thetotal frequencyof 132 drugsinthe treatment of hepatocellular carcinoma in the Miao medicine was147times,mainly in the hot and coldchanneldrugs,accounting for 90.5%of the total frequency.

Drugefficacy statisticsof Miaomedicine in treatingliver cancer

Asshowninfigure5.The results showed that the first three drugs were poison,complement,ventilation and bloodcirculation,accounting for 80.4%of the total frequency.

Figure 1 Distribution of the genus of Miao medicine for the treatment of liver cancer

Figure 2Distribution of drugproperties of livercancerdrugs by Miaomedicine

Figure 3 Distribution of drugs in treating liver cancer in Miao medicine

Figure 4 Distribution of drugs for treating liver cancer in Miao medicine

Figure 5 Distribution ofdrug efficacyintreating liver cancer in Miaomedicine

Discussion

In the literature of traditional Chinese medicine,liver cancer is closely related to such diseases as"fatty gas,liver accumulation,syndromes and lupus",such as"difficultmenstruation"."All pathogeny"contains:"liveraccumulation,fine veins,pain under two flanks...Body without GaoZe,xi twitch,onyx withered black,spring Chai autumn,color green also"[137].In Miao medical records,liver cancer is closely related to"public turtle",its long in abdomen,shaped like a turtle,round and horns,even more fierce,can be a rapid transition by the slow to fast,hot,that hurts the body gas,let the body gradually angular,up to a certain degree after hifu and adjacent parts will produce severe pain,sideburnsvellusmatte,complexion,diet,and dismounted vein and finger vein were quick to or subtle[138-139].It is very similar to advanced liver cancer in modern medicine.Miao medicine believes that liver cancer is a malignant disease,which is formed by damage to liver shelf qi by chemical substances such as primary liver disease,nitrobenzene,aflatoxin,nitrite,etc,and malignant mutation of liver and gallbladder cells.Miao medicine calls it"good flea expensive"[140].At present,the curative effectof hepatocellular carcinoma treatment is unsatisfactory.Therefore,it isveryimportantto seekomnidirectional and multitarget drugsto treat livercancer from the pure naturalmedicinewithlittle toxic and side effects.The purpose of thisstudy is to exploreand analyze the drug applicationlaw ofMiaomedicine in treating livercancerso asto providesome referencefor theclinicalapplication of Miao medicine in treating liver cancer and the development of new drugs.

Analysis ofthepharmacologyforthe treatment of livercancer inMiao medicine:

Genera statistical results according to the distribution,the Miao medicine treatment for liver cancer drug variety,plant drugs in compositae,rosaceae,blanc genera,for high frequency has the inhibitory effect of liver cancer drugs is more,some drug ingredients significant anti-tumor activity,such as compositae thistle,ghost needle grass,einar incense,such as flavonoids,liliaceous plants dwarf lilyturf,steroidal saponins ingredients such as lily,rhizoma polygonati, fructus rosae laevigatae rosaceae plantsin zonetie elm,and Chinese cinquefoil herb of triterpene compounds,have obvious inhibition activity of liver cancer[141-143].Thiscan provide the research direction and literature basis for the screening of clinical drugs forthetreatmentoflivercancerin modern medicine and the development of new drugs.

Drugresistanceanalysisfor treating livercancer in Miaomedicine

According to the distribution ofdrug property statistics,coldandhot drugs wereinvolved inthe treatmentof livercancerin bothsexes of Miao medicine,butcold drugsweremainly used,accountingfor75.8%.Underthe guidanceofthe theoryof twoclasses,Miao medicine takes"cold diseaseheat treatment,fevercold treatment"as the therapeutic principle.Cold medicineis mostly used totreatfever,such as the use of thecold medicine such asrhubarb,sophora flavescens,honeycomb andsoon to treatdysphoricfever,drystools,short of urine,red tongue,yellow moss,pulsehongda and other hot diseases.Therefore,in the Miao medicine,liver cancer isoftenseenas fever.The useofthemedicinal property of the Miao medicine tendstobe consistentwith thatof traditional Chinese medicine,which is to correct the pathological state ofthebody'sexcessive,partial anddecayingbymeansofcoldandhot drugs.Relevant studieshaveshownthatcolddrugs can resist pathogenicmicroorganisms,resist endotoxin,improvethe body'simmunity,removeheatand fire poison,and effectively treat malignant tumors[144].

Analysisofdrug tasteof Miaomedicine in treatinglivercancer

From thedistribution of thestatistical results of drugtaste,the eightmedicinalflavorsofthe Miao medicine for liver cancer wereinvolved,which reflected the emphasisof theMiao medicineon holistic treatment,butalso focusedon syndrome differentiation for treatment.The firstoneis the bitter medicine,which hasthe function of removing hot poison,dryingwet fireand releasing heat.For example,jiujie tea,sanpifeng,sanzhishan,which accounts for 39.8%,is usedin accordance withthe pathogenesis of livercancerdampness-heat and stasis,as stated inthenote of shen zhai death.In the second place is thespicymedicine,whichhasthe effectofdispersing qi,such astiannanxing,yuanbao grass,pine and soon,accounting for 21.2%.Itsusemeetstheconditions forthe formationof liver cancer,that is,qi,blood,water and malignancy.In the third place was the sweet medicine,whichcanhelpthe weak to fill up deficiencyandquench thirsttoproducezins,such as poria cocos,hook vine and eucommia,accounting for 15.2%.

Analysisof Miaomedicine treatment of liver cancer

Accordingto the distribution ofthestatistical resultsoftheregressionanalysis,themajority of thepatientswith HCC treated by the Miao medicine were hot meridians,accounting for 68.7%.In the medicine of miao medicine,it is classified according to the nature and tendency of drug action,and mainly refers to the trend and mode of drug action.And hot classics medicine has the function of clearing heat and detoxification,soft and solid dispersal,purging fire and blood,et al,mainly used for treating a kind of hot diseases that the living things of the body can be hyperactive or the external heat and toxin invade the body.The results of the analysis of hepatic carcinoma with fever were consistent with the statistical results.At the same time,it is also consistent with the heat syndrome where the frequency of hepatocellular carcinoma(HCC)is the highest in TCM[145],and related studies have shown that the increase of total bilirubin is closely related to the pathologic"heat"[146].In addition,31.3%of the drugs were cold menstruation,slow menstruation,fast meridians and half meridians,showing that the medicine for treating liver cancer in Miao medicine could be applied flexibly with different properties,different speeds and different types of drugs according to the patients'conditions and potential diseases.

Analysis ofthe efficacy of Miaomedicine in treatingliver cancer

Accordingto thedistributionof efficacy statistics,thefirst one waspoisontreatment,accountingfor 44.0%.Miaomedicine believes that"poison is the cause of chaos,non-toxic and non-pathogenic",and malignancyis therootcauseoflivercancer[147].Andpoisonslantsex is heavy,effectisstrong,and can undertake effective attack to "vicious".According to the nature,origin and degreeof poison,the Miao doctor choosesdifferent poison to treat,such asattackpoison,gram poison,defeat poison,drivepoison etc.Among them,the most commonly used andmostrepresentativeMiao medicineis to attackpoison,for example,toads have beentakeninternallywith toadstablets,and the hmongfolkhave toad(toad)detoxification methodfor external use.Thelivingtoad is opened toremove internalorgans and appliedto the affectedarea to absorbandremovetoxins[148].The understanding andtreatmentof"malignancy"in Miao medicine issimilar tothatof"cancer toxicity"intraditionalChinese medicine,bothof which usethe method of poison toattack toxins in thebody.Relevantstudies haveshownthat thedrug has cytotoxic effect,which can inhibitthe growth of tumorcells and inducetheapoptosis of tumor cells[149].According toMiaomedicine,the deficiency ofvital energy inthebodyisthe basisof the onsetandprogression ofliver cancer.The treatment USESsuchdrugs as ganoderma lucidum,dendrobium and radixophiopogonisto restore the livingorganism to gooutwithdetoxification,which is echoed before and after the use of sweet drugs.The theory of"reinforcing weakness"in Miao medicine is similar to the theory of"inner deficiency"in traditional Chinese medicine,and all of them are treated with fuzheng peiben.Relevant studieshaveshown thatfuzheng peiben can regulate and enhance the body's immunity,induce tumor cell differentiation,promote apoptosis of tumor cells,and fight against tumor invasion and metastasis[150].In third place was ventilation and blood clots,accounting for 15.2 percent.Miao medicine believes that"the place where the poison gathers is the place where the disease is formed",the virulence and the pathological product produced by the qi,blood and hydrological knot is the key to the generation of liver cancer.According to the study found that bee venom,pinellia,radix aconiti top drug for Miao medicine crossbow solution is an important part of[139],to be able to play a"cure disease,chronic"effect,and pharmacodynamics has been proved that these liquids have analgesic function,inhibiting inflammatory factor[151],and middle-late exist more pain of liver cancer,the application prospect of the treatment of cancerous pain should be considerable.The use of drugs for ventilation and dispersing blood tendsto be consistent with the understanding of"non-general rule pain"in traditional Chinese medicine.Relevant studies have shown that drugs for promoting blood circulation and removing blood stasis and phlegm and dehumidification have effects of improving inflammatory microenvironmentand inhibiting tumor angiogenesis.

To sum up,it has been confirmed that the theory of Miao medicine, medicinal property and"toxicity"of Miao medicine are scientific in the treatment of liver cancer.At the same time,it also reveals the cold and heat differentiation of the Miao medical treatment of liver cancer,and the rational use of complement medicine,ventilation and blood dispersing medicine and other specimens on the basis of poison treatment.

1. Wanqing Chen,KexinSun,Rongshou,et al.Cancer incidence andmortality in China,2014.ChineseJournal ofCancer Research,2018,30:1-12.

2. Editorial committee of Chinese herbals.The Chinese materia medica Miao medicine volume.Guizhou science and technology press,2005:1-600.

3. Tian ZH,Du J,Deng YH.Miao medicine.Ancient Chinese medical books press,2008:27-48.

4. Ni XZ.Study on the extraction,separation and bioactivity of red polysaccharides and alkaloids.Guangxi university,2013.

5. Zhang ZQ,Xing YJ,Hu GQ,et al.Induction of apoptosis of HepG2 cells in hepatocellular carcinoma(HCC).China J Chin Mater Med,2011,36:2145.

6. ZhuDD,Yao SK,Yan JG,etal.Effectof matrine onHepG2 proliferation,cycleand apoptosisinhuman liver cancer cells.Cancer Research and Clinic,2010,22:745-747.

7. Tang YX,SunYW,Liang G.Study on antitumor activity ofwhite wood-tonol extract in vitro.Chinese folk medicine,2014:17-18.

8. SiKK,WuRB,Chen DZ,et al.Inhibitionof Bel-7404of humanlivercancercell lineby ganglion injection. Journal of youjiang nationalitiesmedical college,2012,34:150-152.

9. Liu X,Jl QB.MTT assaywas used to determine the inhibition of HepG2 in hepatocellularcarcinoma.Journal of Harbin commercial university,2012,28:393-395.

10.Wu SH,Gong YM.The antitumor effect and mechanism of the extract of snakberry on H22 mice with liver cancer.Guangdong Medical Journal,2016,37:1300-1302.

11.Wang YN,Zhu XH,Ma H,et al.The essential oil induced caspase-dependent apoptosis of human liver cancer smmc-7721 cells.traditional chinese medicinal materials,2016,39:1124-1128.

12.Gu D.Study on HepG-2apoptosis inducedby tuckahoe cocosand its mechanism.Traditional ChineseMedicineUniversity OfGuangzhou,2005.

13.ChenHC,Chou C K,Kuo Y H,et al.Identification of aproteinkinaseC(PKC)activator, daphnoretin, that suppresses hepatitis B virusgene expression inhuman hepatomacells[J].BiochemicalPharmacology,1996,52:1025.

14.LiuL,Zhao JL,WangJG.Studyon the mechanism ofoleanolic acidinducing G_2/M phase arrest and apoptosisin humanliver cancerBel-7402cells.China JChin Mater Med，2015,24：4897-4902.

15.ShiXY,ZhaoW,Wang TX,etal.Effectof topical rhein on the proliferation and differentiationofhumanliver cancercells smmc-7721.China Healthcare Innovation,2012,07:11-13.

16.Liu SJ.Study on the selection of anti-tumor components and its mechanism.Sichuan University,2006.

17.Sun XE,Cao BY,Chang YQ,et al.Study on anti-tumor effect of polypeptide lysate of jujube.Food science,2008,29(11):597-600.

18.Xu XJ,Cai YX,Mao Yu,et al.Antitumor mechanism of pleurotus polysaccharides on hepatocellular carcinoma(H22)mice.Food research and development,2015:23-25.

19.He ZM,Bai B,WangH,etal.Study on antitumorandantivirus activityin vitro of totalflavonoids of radixfarinosa.Chinese patent medicine,2010,32:2045-2047.

20.HuangXM,LiuYH,DuGJ.Preliminary study on antineoplasticactivityofradix aconiti multiflori.JournalofHenan University,2014:82-84.

21.LiGF,Wu CJ,MaJX,et al.Studyon inhibition of xenograft tumor of human liver cancer cell line inducedbyextractsof p.gierensis.Journal of Nutrition,2008,30:631-632.

22.Wang YS,Luo S.Theantioxidant effect of rubrum anthraquinone onsmmc-7721 cells of liver cancer.ShandongMedical Journal，2010，50：45-46.

23.Cui XS,Jin YZ,Zhang XW.Experimental study on inhibition of HepG-2 in hepatocellular carcinoma cells by purslane extract.Lishizhen Med Mater Med Res,2007,18:1065-1066.

24.Cui L,Song SL,Sun LR.Study on chemical constituents of desquamate and preliminary screening of antitumor activity.traditional chinese medicinal materials，2006，29：703-705

25.Ren LP,Li XJ,LiangSC,et al.Effect of total flavonoidson HepG-2 cellapoptosis in hepatocellular carcinoma.Gerontology in China,2016,36:4721-4723.

26.YangZH,YinJY,WeiZR,et al.Apoptosis of smmc-7721 cellsinduced by tiannanxing extract and its mechanism.Chinese Journal of Gerontology,2007,27:142-144.

27.Wang Q,ZhangY,LiJ,et al.Gastrodin suppresses tumor growth by affectingthe immune system of mice. Journal of immunology,2014:566-568.

28.Tang WH,WuZQ,FanJ,etal.The anticancer effect of trichosanthin protein on hepatocellular carcinoma in nude mice.Chinese Journal of Hepatobiliary Surgery,2003,9:228-230.

29.Zhang MG,Chen G,Liu L.Extraction of aspartic polysaccharides and its effect on the growth of smmc-7721 cells in human liver cancer.Journal of Interventional Radiology,2011,20:465-469.

30.Fan SJ.Study on the preparation and antitumor effect of total alkaloid in the blood of pteroptera of pteroptera.Shanghai University of Traditional Chinese Medicine,2011.

31.Huan LJ.Study on chemical composition and biological activity of ingots.Hubei university,2014.

32.XieMN,ZhuangWX.Invitro,FIG leaf ultrasonic extract induces HepG2 cell apoptosisin liver cancer.Lifescienceresearch,2010,14:523-527.

33.LiDD,LiY.Effect ofhibiscus acetic acidon metastasis of livercancerbel-7402cells.China Practical Medical,2011,6:160-161.

34.Cai XL,GuoY,WangJ,etal.Isolationand purification of chymopapain and its effecton killing liver cancercells in mice.Chinese JournalofBiochemical Pharmaceutics,2004,25:215-218.

35.TangXJ,Huang HR,Wang TZ,et al.Study on theanti-tumor effect andmolecular mechanismofbutanolextract.PharmacolClin Chin Mater Med,2003,19:24-25.

36.Wang B,Zheng GC.Effect of arctiin on invasion and metastasis of liver cancer.World Chinese Journal of Digestology,2011:723-727.

37.Zhao XM,Zhang FG,Shen HS,et al.Comparative study on antitumor activity of 4 extracts from burdock root in vitro.China Pharmacy,2012:1351-1353.

38.WangYu,WangLin,LiuL,et al.Studyon antitumor activityof beefzhi oil invitro.ChineseJournal of Microecology,2010,22:1101-1102.

39.CaiYY.Studyon chemicalcomposition and antitumoractivityof n-butanolin Chinese rose.Zhengzhou University,2014.

40.Sun J.Effectsof astringentson bloodand lymphatic metastasisof liver cancer in mice.TraditChin DrugResPharmacol,2008,19:347-350.

41.RajkumarV,GunjanG,KumarR A,etal.Evaluation of CytotoxicPotential of Acorus calamusRhizome[J].Ethnobotanical Leaflets,2009.

42.Lv DX,Wang XL,Wei FX,et al.Study on the induction of apoptosis of smmc-7721 cells in human liver cancer by maize beard polysaccharide.Heilongjiang Medicine And Pharmacy,2006,29:28-29.

43.Ou CL.Study on the chemical constituents of ethyl acetate and its anti-liver activity.Guangxi Medical University,2017.

44.Li J.Studyonanti-tumor activityand molecular mechanism of pomegranate polyphenol on HepG2 in human liver cancer cells.Shaanxi Normal University,2015.

45.Luo WJ,Wang GH,Zhang X,et al.The inhibition of the proliferation of fhcc-98 in human hepatocellular carcinoma(HCC)was studied.China organizational engineering research，2006，10：150-152.

46.Hao KH,Han T,HuPB.Study onthe inhibition oftumor growthandmechanism by monocrotic acid in mice H22. China Pharmacist,2016,19:29-32.

47.GaoSY,JiZB.Effectsofsolanine on nacetyltransferaseactivity in HepG2 cells of human livercancer.Chineseherbal medicine,2008,39:1688-1691.

48.Pang JX,MaRQ,Liu LM,etal.Toxicityand anti-tumoreffectoftotalalkaloids in liver cancer cells.Journalof SouthernMedical University,2005,25:325-328.

49.SunZY,Jin GJ,XuT,etal.To treat 30 cases of primary livercancer.Journal of integrated Chinese and western medicine,1995(4):29-30.

50.Che YL,Liu SJ.Study on white and treatment of transplanted liver cancer in mice.Information on Traditional Chinese Medicine,2008,25:38-40.

51.Zhang JZ,Wan Q,Liu Y.Effects of leucoquinone on HepG2, smmc-7721 proliferation and the expression of Bax/bcl-2 and Cyclin D1 mRNA in human liver cancer cells.Chinese Pharmacological Bulletin,2012,28:1729-1732.

52.HeSH.(S),6-methyl-3-4(S)-kayforest ester typesynthesis ofcoumarinderivatives and its antitumoractivity.ShanxiMedical University,2016.

53.LiWT,Zhao FM,Dai ZH,et al.Hedyceps sinensis injection inhibited smmc-7721 proliferation and regulated bcl-2 /CytC signaling pathway induced mitochondrial apoptosis.Chinese patent medicine,2015,37:2264-2267.

54.Xu L,Luo H,Yang Y.Screening of endophyticbacteriain ginger foranti-cancer activity.Journal of Henan Agricultural University,2013,47:457-460.

55.Li T, Wang YC. Effect of cactus polysaccharides on growth inhibition of human Bel-7402 liver cancercells.Medical science frontier BBSnationalconferenceon pharmacology and chemotherapy.2011.

56.Zou XH,Zhang KH,Chen J,et al.Anticancer effect and its mechanism of water extract of agrimony on SMMC-7721 hepatocellular carcinoma cells.Chongqing Medicine,2013:3929-3931.

57.Wu JF.Study on chemical constituents and biological activities of two medicinal plants of polygala.Shenyang pharmaceutical university,2007.

58.Huang J,Zhang HY.Effects of pinacel extract on the proliferation and cell cycle of HepG2 cells in human liver cancer.J China Pharm Univ,2009,40:89-93.

59.GaoD,LiuRY,Zhang ZL.Experimental study on the induction ofapoptosisof hepatocellularcarcinoma cellsby calcium signal.Journalof rehabilitation,2006,16:000032-49.

60.ZhangGF,WuLm,Li YB.Preliminarystudy on the anti-liver cancereffectof water extract of xu changqing.ChinaTropical Medicine,2006,25:228-229.

61.Liang QL,GongZN,Xu GL,etal.Studyon anti-tumoreffectof dicholinesesquiterpene lactone invitro.NatProdResDev,2008,20:436-439.

62.BaiMD,YuFR,WangP,etal.Inhibition effect ofcoriander extracton HeP,S180and U14 and immunefunctionof mice.Journalof Practical Oncology,2002,17:117-118.

63.Zhou ZJ,Liu HY,Wang XM.The inhibitory effect of water extract from dioscorea chinensison the expression ofapoptotic protein in hepatocellular carcinoma(HCC).Chinese journal of experimental formulas,2013,19:241-245.

64.Qin SH,Mou YL,Zhou L,et al.Study on the inhibition and mechanism of total saponins on ascites H22 hepatocellular carcinoma in mice.J Chin Med Pharmacol,2013,41:10-13.

65.YangXJ,ZhouK,WeiXQ,et al.Study on the mechanism ofactivating p53-ros pathway inducedapoptosis ofHepG2cells inhuman liver cancer by steroid saponin rce-4.Pharmacol Clin Chin Mater Med,2016:62-67.

66.Zhang D,ZhangM,JiangFL,et al.Effectsof lilium polysaccharideson CyclinD1 andcox-2 cellsin human livercancer.ChinJ Cell Mol Immunol,2011,27:582-584.

67.Shen X.Study on anticanceraction and mechanism oftotalsaponinsincinnabarroot.Beijing University of Chinese Medicine,2003.

68.ZhouZH.Molecular mechanism of HepG2 cell autophagyinduced by ophiopogon B in human liver cancer. The northern pharmaceutical,2017,14:156-157.

69.Zhao XX,Zhang CY,WEN F,et al.Study on apoptosis and molecular mechanism of liver cancer cells induced by sichuan pepper extract.Journal of Modern Oncology,2017,25:14-17.

70.Wei AQ,Li XW,Lian XZ,et al.The inhibitory effect of the drug serum of xanthion on the proliferation of human liver cancer cells.Ecological science,2011,30:647-649.

71.Chen LH,Li X,ChenLihua,et al.Study on chemicalcomposition and pharmacological action of l.chinensis.Asia-Pacific Traditional Medicine,2014,10:33-35.

72.Yuan DX,ShuLX,HuangR.The antitumor effectand mechanism of flavonoids of eucommia ulmoides ii on H22 mice.Gerontology in China,2016,36:291-293.

73.Xu J.Study on the chemical composition and biological activityof thestaves.Zhejiang Gongshang University,2010.

74.ZhuLH,Liu XD,TanYH,et al.Inhibition of growth andinductionof apoptosis of HepG2 in human hepatocellularcarcinoma cells.ChinesePharmacologicalBulletin,2009,25:68-71.

75.Zan JF.Study on antitumoractivityofthree terpenes of poriacocos andquality analysis of poriacocos.HubeiUniversity of Chinese Medicine,2012.

76.Sun XH,Sun YW,Li H,et al.Effects of emodin,emodin and distyrene on hepatocytes and hepatocarcinoma cells.Modern journal of integrated Chineseand western medicine,2010,19:1315-1317.

77.He GZ,Sheng SX,He QS,et al.Experimental study of effects of saponins total flavonoids on proliferation,apoptosis and invasiveness of HepG2 cells in hepatocarcinoma.Journal of Natural Science of Hunan Normal University,2012,35:77-81.

78.Xu J,Wu L,Xu QF.Studyon theinduction of HepG2 apoptosis by ganoderma polysaccharides. Contemporary Chinese medicine,2009,16:7-9.

79.Cha Y,CunYL,HuangYC.Effect of matrine on apoptosis and autophagy related gene expression oflivercancercelllinesmmc-7721.World ChineseJournalof Digestology,2010:3078-3083.

80.ChenSH,ChenXG,He JL,et al.Preliminary observationontheinduction ofapoptosis of hepatocellular carcinomacellsby flavonoids.Anhui Agricultural Sciences, 2015,v.43;No.498:32-34.

81.Dai GH,YangF,Tong YL,et al.The effect of polygonumcuspidatum extract on HepG-2 hepatocellularcarcinoma cellline.China traditionalChinese medicinetechnology,2009,16:376-377.

82.Yang J L,Shen Z M,Sun Y F,et al.[Cultured human hepatoma cells (BEL-7404) for anticancer drugs screening][J].Zhongguo yao li xue bao=Acta pharmacologica Sinica,1985,6:144.

83.Wei YM,Wang S,Meng XS,et al.Study on the efficacy comparison and extraction method of two disinfectants based on the growth inhibition ofsmmc-7721 hepatocarcinoma cells.China Medicine and Pharmacy,2013:35-37.

84.Liu SJ,Li SY,Song JH,et al.Study on the anti-tumor effect of polysaccharide from Chinese gooseberry.Chin J Mod Appl Pharm,2011,28:185-188.

85.HuangDD,ZhangWY.Theinhibitionof HepG2 in humanhepatocellular carcinoma cells anditsmechanism.Journalof Southeast University,2009,28:302-306.

86.HuangYL,LiuY.Study onantitumor activity of polysaccharides in extract of fructus rosae.Genomicsand Applied Biology,2015,34:1848-1851.

87.Cao B.Effectofdrugserumofcarborundum on theexpressionofPOLD1 geneinsmmc-7721 cells ofhumanliver cancer.Guangxi Medical University,2013.

88.LiXL,WuYL,LinZQ,etal.Effect of total saponins on the proliferation andapoptosisof human livercancercellBel-7402.Chinese journalofexperimental formulas,2012,18:238-241.

89.Ma LY,Li L,Jiang Y,et al.Study on the induction ofHepG2 apoptosis in human hepatocellular carcinoma cells by endoplasmic reticulum stress pathway. Chinese Pharmacological Bulletin,2011,27:1523-1527.

90.Cao SQ,Li P,Yin TY,et al.The reversal effect of curcumin on multidrug resistance of human liver cancer resistant cell line bel7402/5-fu.World Chinese Journal of Digestology,2012:135-139.

91.WangYN,CaoR,ZhuC,et al.Study on the effect and mechanism of agp on the angiogenesis of hepatocellularcarcinoma.Chinese JournalofClinicalOncology,2014:758-761.

92.Xu J,LiXB,Lai PJ,etal.Studyon the killing mechanism ofplatycodon saponin D on human hepatocellularcarcinoma cellline HepG2 in vitro.ChinJ HealthLab Technol,2015:2754-2757.

93.Zhang F,HuXB,CaoRY,etal.Studyon anti-tumor effectoftrichosanthin proteinon mice H22.J China Pharm Univ,2011,42:242-246.

94.WuRM,Fang XY,Ling YW,etal.Inhibitory effectof semenloniceraeon liver cancer inducedby diethylnitrosamineinrats.Herald of Medicine,2015,34:463-466.

95.Hu Q,Zhu KJ,Tan XN,et al.Experimental study on the effect of total flavonoids on HepG2 cell proliferation in human liver cancer.Hunan J Tradit Chin Med,2015,31:166-168.

96.Li JH,Zhang XH,Chi HH.Study on the antitumor effect of different extraction methods of xanthospermum. Medical research with education,2000:5-7.

97.LiXW,Wang JJ,LiangST,etal.Effects of extracts ofberberine and glucoside on HepG2 cells.Jilin University,2015.

98.DuanH,Wang BQ,ZhangYW.Studyon the tumor inhibitionand mechanism of flavin polysaccharidein H22transplanted tumor mice withliver cancer.Tradit Chin DrugRes Pharmacol,2014,25:5-7.

99.LouLJ,Lv DL,Hu ZR,etal.Experimental studyon theresistance of kiwifruit to liver cancer.Chinese journal of traditional Chinese medicine,2009:1509-1511.

100.Liu C,Liu SP.The effectsof aglyaponinon HepG2 proliferation and apoptosis in hepatocellular carcinoma cells.Lishizhen Med Mater Med Res,2013,24:2634-2636.

101.Yang XX,Liang GY,Xu BY,et al.Antitumor experiments in vitro on horseshoe gold derivatives.Asia-Pacific Traditional Medicine,2014,10:16-18.

102.Deng Y,LiX,Shao M,etal.Gas chromatography-mass spectrometry analysis and antitumor activity in vitro of essential oil from allelopathic tree.Guiding Journal of TCM,2013:80-82.

103.Wang G,ZhangLM.Study on antitumor activity of extractsof p.cyperus.Journal of the hebei academyof sciences,2008,25:52-54.

104.Lin LQ,Lin XH,Huang LY,etal.Studyonanti-tumor activity in vitroofvetivervetiver extract.J Fujian Med Univ,2010,44:83-85.

105.He S,Pan Y,HuangGK,et al.Inhibitory effect andmechanism ofwater extract from horse foetus onlivercancer.Jiangsu Medical Journal,2018.

106.Zheng L.Studyon chemicalcomposition,structure-activityrelationshipand anti-tumor activity mechanism of acacia chinensis.Shenyang Pharmaceutical University,2004.

107.Chen YH,Zeng Y,Tan YC,et al.Effects of heavy building waterextracton HepG2 roliferation and apoptosis of hepatocellular carcinoma cells. Journal of youjiang nationalities medical college,2013,35:595-597.

108.Chen ZT,Huang ZY,Wu LN,etal.Experimental study of artemisinin-mediated apoptosis of hepatocellular carcinoma cells.Journal of integrated Chinese and western medicine,2000,10:23-25.

109.Feng JD,LIN DH,Liu XQ,et al.The inhibitory effectofacanthopanax on the expression ofvascularendothelial growth factor in human liver cancer cell lines.Tradit Chin Drug Res Pharmacol,2007,18:339-341.

110.Zhou YL,Feng X,Zhu K,et al.Induction of apoptosis in HepG2 human hepatocellular carcinoma cells induced by crude polyphenol from kutin tea.Science and Technolog,2015:339-342.

111.HaoJH,ZhaoCC,LiuMX.Isolationand identification of antineoplastic active components oftrifoliumchinense.Journalof yangzhou university,2011,14:48-50.

112.Zhang MH,WangXY,WangXM,et al.Experimentalstudyon inhibition oflycium barbarum polysaccharides on transplanted liver cancer in mice.Chineseherbal medicine,2012,43:1142-1146.

113.Zhao MY,GuoT,WangMW,et al.The inhibitory effectandmechanismof kurotenac EonHepG2cellsin human liver cancer.Journal of Modern Oncology,2010,18:2091-2094.

114.Ren P.Studyon antihepaticactivity of total flavonoids ofduckweed and itsmechanism in vitro.Chongqing university,2006.

115.Li YC,Yang WJ,Chen Y.Inhibition of the proliferation of hepatocellular carcinoma(HCC)HepG2 cells by lignin 3 and its effect on the protein kinase Akt and erk1/2 signaling pathways.Chinese pharmacy,2013:3099-3101.

116.Jia C.Study on the biological activity and application of ricin.Lanzhou University of Technology,2008.

117.Guo JB,YeHH,Chen JF.Actionand mechanism of dandelion extract against HepG_2 proliferation inhumanliver cancer cells.traditional chinesemedicinal materials,2015,38:2129-2133.

118.MaY.Experimental study on inhibitionof invasion and metastasisofhepatocellular carcinoma cells by the Notch signal pathway.Nanjing medical university,2016.

119.LanM,Lin Y,Zhang RT,etal.Effect of total flavonoids oftarantula on anti-tumor effectof HCC (22)miceand pathways in cancer.Chinese journal of traditional Chinese medicine,2014:1006-1008.

120.Ma P,Cao TT,GuGF,etal.The mechanism ofinducingapoptosisofhuman liver cancer cell lineBel-7402 bysynthesisofaubergine saponin.ChineseJournalofCancer,2006,25:438-442.

121.Song WC,He XL,Lu C,et al.6'-hydroxylactin A induces apoptosis of HepG2 cells in human livercancerand its mechanism.Chinese journal of pharmacology and toxicology,2015:220-226.

122.Zhao MF,Liu YP,Jin B,et al.Experimental study on the efficacy and side effects of toadoxin on mice bearing tumor in H22.Chinese journal of cancer prevention and control,2005,12:1705-1709.

123.YanYQ,Zhao XC,DuYP,et al.Effects of drug-containing serumsofbombyx batrytici on the invasiveness oflivercancer cells.JournalofTraditionalChinese Medicine,2018:156-159.

124.JiaAM,Hu WM,ZhangH,et al.The effect of honeycomb extract on H22liver cancer and its mechanism in mice. World journal of integrated Chineseand western medicine,2012,7:1045-1047.

125.YangGQ,Zhang CG,Zi JJ,etal.Studyon cytotoxicityof threehuman hepatocellular carcinomacell linesusinganOrientalmole cricket extract.Chinese folkmedicine,2017,26:66-69.

126.LiSG.Study on cellcycle arrestand mechanism ofhuman livercancercells induced bymelittin.SecondMilitaryMedical University,2010.

127.Zhao JY,Liu LY,Shen AL,et al.Effect of bear bile powder on STAT3 pathway of liver cancer transplanted in nude mice.Chinese Journal of Integrative Medicine,2014,34:976-981.

128.Liu XP,Zhong DW.Study on the inhibitory effect and mechanism of centipede extract on transplanted liver cancer in nude mice.Chinese Journal of General Surgery,2010,19:164-168.

129.Chang CX,Chen H,Jihong,etal.Experimental studyonthe anti-metastasis of earthwormfibrinolyticenzyme.Tradit Chin Drug Res Pharmacol,2009,20:520-524.

130.ZhangXX.Study on anti-livercancer of softshelled soft-shelledturtle.Yangzhou university,2011.

131.Zhao HC.Gallate induces apoptosis of smmc-7721 cellsinlivercancer.Gerontologyin China,2010,30:3728-3729.

132.Hou XH,Sun Y,LiXF.Effects of chloromethaneleaching on theproliferation andcycle of twocancercells.Chinese patent medicine,2012,12:2278-2281.

133.Huang JJ,Dai ZK.Realgar action against human liver cancer BEL-7402 cells.Journal of Chongqing Medical University,2011,36:936-939.

134.Zhao Q,Zhao M,Lu J,et al.Effects of total anthraquinone of cinnabar on proliferation and apoptosis of smmc-7721 cells in human liver cancer.Journal of tai shan Medical College,2013,34:245-247.

135.Lu G,Wang JS,LU ZL,et al.Nan Jing Ji Zhu.Liaoning science and technology press,1997:44-44.

136.Chao YF.General Treatiseonthe Causeand SymptomsofDiseases.Liaoning Scienceand Technology Press,1997:7-97.

137.Wang FL,Liu F,Leng Y.Onunderstandingof cancerinMiaomedicine．Paper compilation ofthe academic exchangemeetingof anorectal societyof integratedChineseand western medicinesociety of guizhou province and the newprogress ofnew technology． Zunyi:Guizhou integrated Chinese and western medicine society,2012:112-113．

138.DuJ,Zhang JM.Miao medicinefoundation.Beijing:Ancient Chinesemedicalbooks press，2007：281-285.

139.Tian XX.Chinese Miao medicine.Guizhou science and technology press,2013：231-231.

140.Zhao M,Zhou SQ.Research progress on the anti-tumor effect and related mechanism of flavonoids.China Pharmaceuticals,2011,20:1-3.

141.Liu X,Yu JL,Liu M,et al.Progress in the study on the biological activity of steroid saponins in recent 10 years.China J Chin Mater Med,2015,40:2518-2523.

142.Su SL,LiYH.Study on antitumor activityof triterpenoidsaponins in traditionalChinese medicine.LishizhenMed Mater Med Res,2006,17:1153-1155.

143.LiuLL,Chen J,ShiYP.Research progress of antitumor effect of antipyreticand detoxifying traditionalChinesemedicine.Chinese herbal medicine,2012:1203-1212.

144.Li YT,Wu TY,Zhu L,etal.Pathologic characteristics of syndrome element in primary liver cancer.Forum onTraditional Chinese Medicine,2014,29:24-25.

145.Shi HY,WuCY.Thecorrelation between location, pathogenicity and objective indicatorsof primaryhepatocellular carcinoma basedon associationrules.Guiding Journalof TCM,2016:38-40.

146.Shui HL,Yang Z,Cui J,et al.Differences and similarities in the treatment of cancer pain by miao and TCM.Journal of Traditional Chinese Medicine,2017:2158-2160.

147.Du J,Deng YH,Yang HJ.Chinese miao medical secrets. Guizhou science and technology press,2014.10-193.

148.WangXX,Zhou ZW.Progressinresearchon theanti-tumoreffectoftraditionalChinese medicine with poison.Herald of Medicine,2008,27:1364-1366.

149.Hua HQ.Modern research on the treatment of malignant tumors by fuzheng peiben.China J Chin Mater Med,2008,33:1094-1097.

150.Liu XY. Experimental study on the acupuncture therapy of Miao medicine crossbow.Guiyang: Guiyang college of Chinese medicine,2009.

151.ChengHB,ShenZJ,SunDD,et al.Comment ontheeffect of anti-tumorChinese medicine on tumor microenvironment. Journal of Traditional ChineseMedicine,2014,55:1343-1346.