Protective effect and its mechanism of codonopsis pilosula polysaccharide on memory consolidation disorder induced by cycloheximide in mice

Wei LI,Wei-da LIU,Li-yao DUAN,Jiang-man LI,Dan-shen ZHANG

(1.Department of Pharmacy,Hebei North University,Zhangjiakou 075000,China;2.Key laboratory in neuropharmacology of hebei province,Zhangjiakou 075000,China;3.Peking Jabrehoo med tech co,ltd,Beijing 102629,China;4.Hebei University of Science and Technology,Shijiazhuang 050018,China）

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Protective effect and its mechanism of codonopsis pilosula polysaccharide on memory consolidation disorder induced by cycloheximide in mice

Wei LI1,2,Wei-da LIU3,Li-yao DUAN1,Jiang-man LI1,Dan-shen ZHANG4

(1.Department of Pharmacy,Hebei North University,Zhangjiakou 075000,China;2.Key laboratory in neuropharmacology of hebei province,Zhangjiakou 075000,China;3.Peking Jabrehoo med tech co,ltd,Beijing 102629,China;4.Hebei University of Science and Technology,Shijiazhuang 050018,China）

OBJECTIVETo investigate the protective effect of Codonopsis Pilosula Polysaccharide(CPPS)on improving of the memory consolidation disorder induced by Cycloheximide and its possible mechanisms in mice.METHODSThe mice was divided into five groups,as normal control group,cycloheximid model group,piracetam positive control group,CPPS 300 mg·kg-1group,and CPPS 150 mg·kg-1group.The mice respectively were given saline,piracetam,and CPPS for 15 d.The memory consolidation disorder model in mice was established by ip.Cyclohexylamine,and orally administered CPPS(300 mg·kg-1or 150 mg·kg-1)every day.Then experimental groups were subjected Morris Water Maze test.Western blotting analysis were used to analysis the expression of CaMKⅡ/CREB signaling pathways.RESULTSMorris water maze experiment showed that cyclohexylamine can cause memory consolidation disorder(P＜0.01),and giving piracetam and CPPS(300 mg·kg-1)can improve spatial memory impairment in mice(P＜0.05,P＜0.01).Western blotting experiment results show that compared with normal control group,CaMKⅡand CREB contents of brain in model group mice had significant decreased(P＜0.001);Compared with model group,CaMKⅡ and CREB contents of brain tissue in piracetam and CPPS groups increased significantly（P＜0.05，P＜0.01，P＜0.001）.CONCLUSIONCyclo⁃heximide can induce the memory consolidation disorder,and its effect in mice related to CaMK/CREB signaling pathways.CPPS can improved this memory disorder by influence CaMKⅡ/CREB signaling pathways.

codonopsis pilosula polysaccharide;cycloheximide;memory consolidation disorder;Morris water maze;CaMKⅡ/CREB signaling pathways

The project supported by National Natural Science Foundation（81202192）;and Fund of Hebei Provincial Health Bureau（20130037）

Dan-shen ZHANG,Tel:18903139966,E-mail：zhangds2011@126.com