Effect of Shenfu injection on microcirculation effect index in early-and middle-stage of cardiogenic shock rats

Li JIANG,Lan-bin YU,Xiao-Jun YAN,Yu CHEN,Rong YAO,Guo-liang XU

(1.Research Center for Differentiation and Development of TCM Basic Theory,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;2.Jiangxi Provincial Key laboratory of TCM etiopathogenisis,Nanchang 330004,China）

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Effect of Shenfu injection on microcirculation effect index in early-and middle-stage of cardiogenic shock rats

Li JIANG1,2,Lan-bin YU1,Xiao-Jun YAN1,2,Yu CHEN1,Rong YAO1,Guo-liang XU1,2

(1.Research Center for Differentiation and Development of TCM Basic Theory,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;2.Jiangxi Provincial Key laboratory of TCM etiopathogenisis,Nanchang 330004,China）

OBJECTIVEShenfu injection(SFI)is an effective treatment of cardiogenic shock,the pathology of the central link was microcirculation disturbance.However,whether the microcirculation status of the early-and mid-stage of cardiogenic shock has any difference is unclear.This study aimed to observe the effect of SFI on the microcirculatory disturbance in mesentery for early-and mid-stage of cardiogenic shock rat.METHODSThe early-and mid-stage model of cardiogenic shock was estab⁃lished by ligating the ending or root of left anterior descending coronary arteries(LADCA).The rats were randomly divided into 9 groups,ie control group,early-stage model group,mid-stage model group,3 early medicated groups and 3 mid medicated groups(the dosage was 1,3.33,10 mL·kg-1SFI for cardiogenic shock rats of early-and mid-stage,respectively).Parameters in mesenteric microcircu⁃lation,such as velocity of RBCs in venules,diameters of venules,the count of leukocyte adhesion and vascular permeability which calculated by FITC-dextran leakage were observed through an Geneandi-M2 inverted intravital microscope and high-speed video camera system.RESULTSThe cardiogenic shock induced by ligating the LADCA resulted in a number of responses in microcirculation,including a significant increase in the counts of adhesive leukocytes,narrowing of the vascular diameter,decrease in the velocity of RBCs and dextran efflux.All of the above parameters for early-stage cardiogenic shock rats were attenuated by the treatment with SFI,especially the dosage of 10 mL·kg-1.While SFI had no apparent time-effect on the vascular diameter and vascular permeability in mesentery for mid-stage cardiogenic shock rats.CONCLUSIONThe microcirculation status of the early-and mid-stage of cardiogenic shock rats were quite different.The efficacy of early treatment with SFI was more obvious than the mid administration,which could provide experimental and theoretical basis for the patients with cardiogenic shock in an earlier time.

Shenfu injection;microcirculatory;early-stage;mid-stage;cardiogenic shock

The project supported by National Natural Science Foundation(2010CB530603);Science and Technology Development Plan of Lianyungang City（ZD1508);Natural Science Foundation of Jiangxi Province(20151BAB215037);and Science and Technology Program of Health Department of Jiangxi Province(2015A039)

Guo-liang XU,Tel:15007005811,E-mail：xuguoliang6606@126.com