玉米骨干自交系深度重测序及有利基因挖掘

金危危 赖锦盛 张祖新 高世斌

摘 要：通过我国玉米骨干自交系的全基因组序列图谱、转录组图谱、基于基因的SNP单倍体型图谱等玉米基因组的研究以及玉米重要农艺和经济性状的全基因组关联分析，批量鉴定和发掘玉米重要性状的功能基因，推动我国玉米的基础研究和分子育种研究，最终促进我国玉米产业的发展。对10份我国典型优良玉米自交系全基因组进行重测序分析以及100份典型温带玉米自交系籽粒发育转录组序列分析，分析我国典型温带玉米自交系基因组的多样性和遗传结构；挖掘玉米自交系籽粒发育转录组的特征；构建玉米高密度的基于基因的SNP单倍体型图谱和表达谱（eQTL）；结合关联分析等方法批量鉴定和发掘影响玉米重要性状如品质、抗病性等的重要基因，明确这些基因的功能、作用机理、各基因的优良等位基因及其玉米种质材料，开发一批可用于玉米分子育种、效应明显的功能标记。

关键词：玉米 自交系 重测序 关联分析

Abstract：Through maize genome research （such as our country maize elite inbred lines whole genome sequencing， the transcriptome map， the SNP haplotype map） as well as Genome-wide association study of maize important agronomic and economic characters， this projects aims at identifying and exploring the function gene of maize important characteristics， promoting maize basic research and molecular breeding research， so as to accelerate the development of maize industry in China. Through 10 maize elite inbred lines whole genome resequencing analysis and 100 typical temperate maize inbred lines kernel development transcriptome sequences analysis， we analyzing the genome diversity and genetic structure of the typical temperate maize inbred lines in our country； exploring the feature of kernel development transcriptome of maize inbred lines； constructing maize high-density SNP haplotype map and expression spectrum （eQTL）； identifying the function gene of affecting maize important traits such as quality， disease resistance combining with correlation analysis methods； grasping the function of these genes， mechanism， good alleles and maize germplasm materials； and developing a batch of effect obvious function markers using for maize molecular breeding.

Key Words：Maize；Inbred line；Resequencing；Association Analysis

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