视神经损伤的修复与保护机制研究年度报告

徐颖 阮奕文 罗焕敏 陆大祥 钟敬祥

摘 要：该研究计划通过分子生物学技术、神经电生理技术、组织化学与免疫组化方法以及视觉行为学检测方法，研究视神经损伤的病理及修复机制，探讨纳米材料、神经营养因子、新型Nogo受体抑制剂及抗炎因子等对视神经损伤的修复与保护机制，防治视神经及节细胞的损伤，为视神经假体的成功应用奠定基础。该年度主要在以下几个方面开展了研究：（1）探索微电极刺激视神经的安全电流强度范围、刺激时间时程；（2）测试电极假体插入视神经后的缺血状态以及缺血对视网膜的影响；（3）探索3，4-二羟基苯甲酸甲酯（MDHB）对氧化应激所致RGC-5细胞凋亡的拮抗作用及其作用机制；（4）探索原花青素对视网膜节细胞的保护作用及其作用机制；（5）探索EphA2基因在视网膜神经节细胞损伤中的作用。

关键词：视神经 神经节细胞 修复与保护

Abstract：This project plans to explore the repair and protection of optic nerve after its damage， through molecular， electrophysiological， histological， immunological and behavioral test. The nana-material， neural nutrition factors， new Nogo receptor inhibitors and anti-inflammation factors will be applied to test their effects on the protection of optic nerve， in order to prevent the optic nerve damage and the death of retinal ganglion cells. The ultimate object is to provide foundation for the clinical application of optical nerve prosthesis. In this year， we developed the following researches：（1）Explored the safe range of the electric intensity and stimulating duration of micro-electrode inserted into the optic nerve.（2）Explored whether the insertion of micro-electrode would cause ischemia in optic nerve and how temporal ischemia in the brain affected the histology and function of retinal neurons and the visual behavior of the animal.（3）Explored the protection effect of MDHB on oxidative-stress injured RGC-5 cells and its mechanism.（4）Explored the protection effect of oligomeric proanthocyanidin on retinal ganglion cells against oxidative stress induced apoptosis in vitro.（5）Explored the function of EphA2 in the protection of retinal ganglion cells.

Key Words：Optic nerve； Retinal ganglion cell； Repair and protection

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