植物学

2015-10-29 08:13:26
中国学术期刊文摘 2015年7期
关键词:植物学出版物来源

植物学

来源出版物:Journal of Integrative Plant Biology, 2014, 57(1):93-105联系邮箱:Yuuki Sakai, yuukis0110@gmail.com

封面介绍:Actin-dependent chloroplast anchoring is crucial to maintain specific distribution patterns of chloroplasts.Using a centrifugation assay, Sakai et al.(pp.93-105) demonstrate for the aquatic angiosperm, Vallisneria sp., that an initial step of chloroplast movement in response to high-intensity blue light is loss of anchorage from the plasma membrane.In addition, they clone photoropin 1 & 2, the putatively responsible blue-light photoreceptors, increasing the utility of this experimentally tractable plant for studies of chloroplast motility.

Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells

Yuuki Sakai, Shin-ichiro Inoue, Akiko Harada, et al.

In the outer periclinal cytoplasm of leaf epidermal cells of an aquatic angiosperm Vallisneria, blue light induces “chloroplast de-anchoring”, a rapid decline in the resistance of chloroplasts against centrifugal force.Chloroplast de-anchoring is known induced within 1 min of irradiation with high-fluence-rate blue light specifically, preceding the commencement of chloroplasts migration toward the anticlinal cytoplasm.However, its regulatory mechanism has remained elusive, although pharmacological analysis suggested that a calcium release from intracellular calcium stores is necessary for the response.In search of the responsible photoreceptors, immunoblotting analysis using antibodies against phototropins demonstrated that cross-reactive polypeptides of 120-kDa exist in the plasma-membrane fraction prepared from the leaves.In vitro phosphorylation analysis revealed that 120-kDa polypeptides were phosphorylated by exposure to blue light in a fluence-dependent manner.The blue-light-induced phosphorylation activity was sensitive to a Ser/Thr kinase inhibitor, staurosporine, and unusually was retained at a high level for a long time in darkness.Furthermore, phototropin gene homologs(Vallisneria PHOTOTROPIN1 and PHOTOTROPIN2) expressed in leaves were isolated.We propose that calcium-regulated chloroplast de-anchoring, possibly mediated by phototropins, is an initial process of the blue-light-induced avoidance response of chloroplasts in Vallisneria.

Avoidance response of chloroplasts;blue-light response;chloroplast de-anchoring;cytoplasmic calcium;phototropin

猜你喜欢
植物学出版物来源
将来吃鱼不用调刺啦
再论《山海经·五藏山经》的植物学
古今农业(2021年3期)2021-11-20 06:03:42
数据库在植物学教学中的应用
试论《说文》“丵”字的来源
“赤”的来源与“红”在服装中的应用
流行色(2018年11期)2018-03-23 02:21:22
2017年出版物
Global analyses of sea surface temperature, sea ice, and night marine air temperature since the latenineteenth century
Arctic sea ice decline: Faster than forecast
植物学
2015 年出版物