耐碱转GsGSTs基因苜蓿的农艺性状调查

吴婧，朱延明，王臻昱，柏锡，纪巍，才华

摘要：为研究前期获得的耐碱转GsGSTs基因苜蓿与其对照在农艺性状上是否存在差异，进行了产量性状和形态性状的调查与统计分析。产量性状包括鲜草产量、干物质产量及茎分枝数；形态性状包括株高增长速度、节数及节间长度，同时进行了碱胁迫下表型分析。结果表明，转GsGSTs基因苜蓿植株在产量性状及形态性状方面与对照相比无显著差异。在碱胁迫下，非转基因苜蓿对照株系明显萎蔫、失绿，而转GsGSTs基因苜蓿株系均能保持较正常的生长状态。此结果说明外源基因GsGSTs在苜蓿中的表达没有改变其本身优良的农艺性状，同时其目标性状（耐碱性）有所提高，符合转基因植物安全评价中“实质等同”原则。

关键词：转基因苜蓿；GsGSTs基因；耐碱；农艺性状

中图分类号：S541+.9 文献标识码：A 文章编号：0439-8114（2013）05-1105-04

Investigation and Analysis on the Agronomic Traits of Alkaline Tolerance Transgenic Alfalfa with GsGSTs Gene

WU Jing，ZHU Yan-ming，WANG Zhen-yu，BAI Xi，JI Wei，CAI Hua

（Plant Bio-Engineering Laboratory， College of Life Sciences， Northeast Agricultural University， Harbin 150030，China）

Abstract： In order to study the agronomic performance differences among transgenic alfalfa lines and the control， the yield traits， morphological traits of transgenic alfalfa lines were investigated and analyzed. The yield traits included the number of branches， grass yield and dry weight. And the morphological traits included the growth rate in spring and fall， plant height， the number of nodes and the node length. And the transgenic alfalfa lines were treated with NaHCO3. The results showed that there was no significant difference between transgenic alfalfa lines and non-transgenic alfalfa plants in the yield traits and morphological traits. And it was found that transgenic alfalfa lines grew well in the presence of 100 mmol/L NaHCO3， while wild-type plants expressed chlorosis and wilting The results indicated that the GsGSTs genes could enhance alkaline resistance and its expression in alfalfa had no unfavorable effects in agronomic traits. All the results complied with the principle of “substantial equivalence” of transgenic plant safety evaluation

Key words： transgenic alfalfa； GsGSTs gene； tolerance to alkaline-stress； agronomic traits