MicroRNA-200b调节口腔鳞癌E-钙黏着蛋白的研究

张轶　王寅　吴剑　李龙江

[摘要] 目的 通過口腔鳞癌细胞中过表达microRNA-200b，检测口腔鳞癌细胞E-钙黏着蛋白表达是否有所上调;并检测E-钙黏着蛋白表达上调对口腔鳞癌细胞生物学特性的影响。 方法 通过目的基因过表达慢病毒载体构建（has-miR 200b-LV），转染舌癌细胞株Tca8113，检测转染后舌癌细胞株Tca8113的E-钙黏着蛋白的表达及转染后舌癌细胞株Tca8113的侵袭和转移情况。 结果 实验组通过Real-time PCR数值分析显示miR-200b基因表达定量分析平均值比较，组间差异有统计学意义（P<0.05）;转染后的Tca8113细胞，通过Real-time PCR数值分析显示实验组中E-cadherin基因表达定量分析平均值比较，组间差异有统计学意义（P<0.05）;Transwell侵袭实验中，空白组和对照组穿膜细胞数分别为（29.3±4.5）和（20.2±3.3），与实验组（4.3±2.1）比较，差异有统计学意义（P<0.05）;生长曲线显示microRNA-200b过表达病毒感染的肿瘤细胞生长速度明显减慢。 结论 microRNA-200b的表达可以调控E-钙黏着蛋白的表达，E-钙黏着蛋白的高表达可以抑制肿瘤的侵袭和转移。

[关键词] microRNA-200b;E-钙黏着蛋白;侵袭;转移

[中图分类号] R739.85          [文献标识码] A          [文章编号] 1674-0742（2020）10（a）-0033-03

microRNA-200b Regulating E-cadherin in Oral Squamous Cell Carcinoma

ZHANG Yi1，2， WANG Yin2， WU Jian2， LI Long-jiang2

1.Department of Stomatology， Zhongshan Hospital， Xiamen University， Xiamen， Fujian Province， 361004 China; 2.Surgery of Head and Neck Oncology， West China Hospital of Stomatology， Sichuan University， Chengdu， Sichuan Province， 610041 China

[Abstract] Objective To detect whether the expression of E-cadherin in oral squamous cell carcinoma cells is up-regulated by overexpressing microRNA-200b in oral squamous cell carcinoma cells; and to detect the effect of up-regulation of E-cadherin expression on the biological characteristics of oral squamous cell carcinoma cells influences. Methods The target gene overexpression lentiviral vector （has-miR 200b-LV） was constructed and transfected into the tongue cancer cell line Tca8113. The expression of E-cadherin in the tongue cancer cell line Tca8113 after transfection and the tongue cancer cell line after transfection were detected. Results The real-time PCR numerical analysis of the experimental group showed that the average value of miR-200b gene expression quantitative was compared， and the difference between the groups was statistically significant （P<0.05）; the transfected Tca8113 cells passed Real-time Time PCR numerical analysis showed that the average value of E-cadherin gene expression quantitative analysis in the experimental group was （compared）， and the difference between the groups was statistically significant （P<0.05）; in the Transwell invasion experiment， the blank group and the control group had permeabilized cells numbers were （29.3±4.5） and （20.2±3.3）， which were statistically different from the experimental group （4.3±2.1） （P<0.05）; the growth curve showed the growth rate of microRNA-200b overexpression virus-infected tumor cells was significantly slowed down. Conclusion The expression of microRNA-200b can regulate the expression of E-cadherin， and the high expression of E-cadherin can inhibit tumor invasion and metastasis.

综上所述，microRNA-200b的表达可以明显上调E-钙黏着蛋白的表达， E-钙黏着蛋白的高表达可以抑制肿瘤的侵袭和转移。

[参考文献]

[1]  李加丽.癌症患者生命质量的影响因素及护理干预的研究进展[J].医疗装备， 2017，30（14）：187-188.

[2]  王清富. 溶血磷脂酸通过调控PI3K/AKT信号通路对骨肉瘤细胞黏附和迁移的作用[J]. 肿瘤学杂志，2019，25（4）：320-324.

[3]  Gan W J ， Wang J R ， Xiao-Li Zhu.et al. RARγ-induced E-cadherin downregulation promotes hepatocellular carcin oma invasion and metastasis[J].Journal of Experimental & Clinical Cancer Research， 2016， 35（1）：164.

[4]  Kurata A， Yamada M， Ohno SI，et al. Expression level of microRNA-200c is associated with cell morphology in vitro and histological differentiation through regulation of ZEB1/2 and E-cadherin in gastric carcinoma[J].Oncology Reports， 2018.39（1）： 91-100.

[5]  陸旭， 周闯， 李仁锋， 等. Kindlin-2诱导胆囊癌细胞上皮间质转化促进其侵袭转移的实验研究[J].中华外科杂志，2018，56（8）：617-622.

[6]  刘莉，杨永姣，陈业刚，等.细胞粘附分子-1抑制膀胱癌细胞侵袭转移的机制研究[J].重庆医科大学学报，2018，43（4）：40-43.

[7]  Yue Hu，Yayuan Zheng，Mingrui Dai，et al. G9a and HDAC s are crucial for Snail2﹎ediated Eadherin repression and metastasis in hepatocellular carcinoma[J].Cancer Science， 2019，110（11）： 3442-3452.

[8]  Li C， Liu J， Zhang Q， et al. Upregulation of E-cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder cancer cells by inhibiting β-catenin/TCF target genes[J].International Journal of Oncol ogy，2018，52（6）：1815-1826.

（收稿日期：2020-07-05）