Preface Cyanobacteria and cyanotoxins: responses and detection

LI Renhui (李仁辉) SONG Lirong (宋立荣) Philip T. ORR

1 Key Laboratory of Algal Biology, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

2 Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

3 Australian Rivers Institute, Griffith University, 170 Kessels Rd, Nathan, Qld 4111, Australia

The 10thInternational Conference on toxic cyanobacteria (ICTC-10) was successfully held during 23–28 Oct. 2016. We were so glad to see much progress made on toxic cyanobacteria and cyanotoxins during past years, and the ICTC does provide a global forum for a wide-ranging communication and discussion of key issues related to cyanobacterial blooms and cyanotoxins.

This special issue “Cyanobacteria and cyanotoxins:responses and detection” in Journal of Oceanology and Limnology includes a collection of twelve papers focus on different topics and approaches on diversity,detection and physiological responses of cyanobacterial blooms and cyanotoxins. Seven papers include physiological responses of toxic cyanbacteria to environmental factors. As presented in the keynote speak at ICTC-10, Burford et al. (2018) provided the recent progress on studies about physiological responses to nutrients by toxic Cylindrospermopsis raciborskii, a widespread species with a high level of flexibility with respect to light and nutrients, and they summarized that an important component of this flexibility appears to be the result of within-population strain variability in responses to nutrients, as well as key physiological adaptations.

Peng et al. (2018) deal with the response of Microcystis aeruginosa to different nutrient ratios and changes in phosphorus chemistry, and their results demonstrated the effects of different phosphorus chemistries and N:P ratios on the cyanobacterial growth, photosynthetic activity and cell physiology,which could be an effective tool for predicting cyanobacterial dominance or N-de ficiency in natural lakes.

Huang et al. (2018) investigated the effect of light availability on the speci fic density, size and sinking loss of Anabaena flos- aquae and Scenedesmus obliquus, and the results implied that the sinking loss rate of the two algal species was not always constant but should be considered as a variable response to the change of light availability.

Orr et al. (2018) reviewed the importance of assessing changes in cyanotoxin cell quotas (Q) in response to external stressors, and they showed how first-order rate kinetics at the cellular level can be used to explain how environmental conditions drive changes in bloom toxicity at the ecological level. In summary, those stressors drive changes in bloom toxicity through the effect they have on the rates of cell division and growth of different strains with genetically different cyanotoxin cell quotas, and not through any stimulatory or trigger effect on the toxin production pathway itself.

Wang et al. (2018) provided an exceptional example for the study of regime shift during the last 50 years in Lake Dianchi (China), a plateau shallow lake. The shift, characterized as the dominance of submerged macrophyte into the dominance of phytoplankton (mostly cyanobacteria), has occurred since 1993, and they concluded that the threshold concentrations at which regime shift occurred were total nitrogen (TN) and phosphorus (TP) reaching 1.2 mg/L and 0.13 mg/L, respectively.

Rozina et al. (2018) detected the effect of electrochemical oxidation on Microcystis aeruginosa appling the combination of two sensors as a useful tool for in-vivo detection of induced stress, providing real-time information needed for the evaluation of the efficiency of the in-lake treatment and decision upon the necessity of its repetition. The resuts showed that the electrochemical treatment also resulted in a lower free microcystins concentration compared to control.

Dai et al. (2018) prepared powdered activated carbon (PAC) modi fied with Fe(III) as PAC-Fe(III)and utilized it for the fast and efficient removal of microcystins from water. PAC-Fe(III) exhibited superior microcystin-LR removal capacity and efficiency compared to the unmodi fied PAC. The MCLR removal efficiency of PAC-Fe(III) increased with decreasing pH within the pH range of 4.3 to 9.6, and PAC-Fe(III) could be further reused, suggesting that Fe modi fied PAC is a promising material for the treatment of MC-contaminated waters.

Next, five papers in this special issue deal with the detection of bloom forming cyanobacteria and cyanotoxins in waters. Mckay at al. (2018) presented a toxic Planktothrix agardhii bloom detected in the Maumee River, the largest tributary to Lake Erie during nearly one week between May–June 2016,with highest MC-LR reaching over 22 μg/L. This early onset, microcystin-producing bloom provided a rare opportunity to shed insights into environmental factors that promote bloom development and dominance by Planktothrix in lotic environments.

Han et al. (2018) reported spatial and temporal variations in cyanobacteria and microcystins in Aha Reservoir, Guizhou province, southwest China. As a representative of drinking water resource, this reservoir suffered from annual cyanobacterial blooms dominated by Microcystis sp., mixing with Aphanizomenon flos- aquae and Pseudanabaena limnetica, and Microcystin levels were between 0–3.0 μg/L. The results of this study indicate that the reservoirs used for drinking waters in this region should be monitored regularly to minimize potential health risks to the human population.

Zuo et al. (2018) evaluated different mcy genes for detecting the toxic to non-toxic Microcystis ratio in the field by multiplex qPCR since molecule biological detection for microcystin producing cyanobacteria has been largely performed, and they indicated that mcyD was the most suitable mcy gene for quanti fication in laboratory experiments compared to mcyB which abundances were always higher. These results will help to choose mcy regions for conducting real-time PCR to detect toxic cyanobacteria in waters.

Huo et al. (2018) who used high-throughput sequencing of 16S rDNA to investigate the aquatic bacterial community structure along the water course from Yuqiao Reservoir to Haihe River in Tianjin,China during a bloom in 2016. They found distinct bacterial communities associated with Microcystis vs Synechococcus dominated waters respectively, and applying high throughput sequencing could offer a wide field of vision in analyzing microbial community structures.

Final paper in this collection written by Shen et al.(2018) who used the polyphasic approach to characterize a coccoid cyanobacterium Merismopedia tenuissima. This paper seemed not deal with the toxic cyanobacterial taxa, but the Merismopedia species are commonly found in aquatic environments with different trophic states, and some species were even found as the dominant species as a bloom state in the eutrophic water. Polyphasic characterization on the M. tenuissima strains brought some addition to the general view and basic knowledge for the classi fication and systematics of the genus Merismopedia, and further enhanced the monitoring capacity for unicellular non- Microcystis dominated blooms.

We thank Journal of Oceanology and Limnology editor Roger Z. YU for giving us the opportunity to edit this special issue and supervising the whole editorial process, and we also thank all reviewers that have thoroughly reviewed these manuscripts. We hope that this collection of papers will be of interest for a broad scienti fic audience and will provoke new ideas and new research programs from a wide range of water bodies.