高粘度快速发酵生产燃料乙醇技术研究最终报告

靳艳玲 赵海 方扬

摘 要：在粘度产生机制方面，以甘薯为模式原料利用多糖单克隆抗体芯片分析技术解析了粘度产生的机制，为降粘技术的开发提供了靶点；在降粘技术方面，利用商品化酶开发了复合降粘酶系，同时还自主筛选出了可以同时产生复合降粘酶的单菌株，并开发了配套的降粘工艺，可使甘薯、木薯、芭蕉芋等粘度下降90%以上；在菌种选育方面，选育到8株高浓度乙醇发酵酵母，并通过适应性驯化提高了菌株对高温、高压等环境压力条件的抗性，同时还研究了菌株的压力耐受机制，为进一步改造或优化其应用工艺提供数据支持；在发酵过程调控方面，开发出了代谢促进剂及配套的高浓度快速乙醇发酵技术，发酵24 h内，乙醇浓度可达12%以上；在反应器开发方面，开发出新型利用变频泵驱动的高传质低能耗反应器，可使粘度高达9万mPa·S的薯类浓醪传质均匀；通过高效乙醇发酵菌株，降粘技术体系，快速乙醇发酵和高浓度乙醇发酵等关键技术模块的系统集成建立了高粘度快速发酵生产燃料乙醇技术体系，并应用于西南地区最大的乙醇生产企业——资中县银山鸿展工业有限责任公司3万t燃料乙醇示范生产线上。以鲜甘薯为原料，发酵时间由现有技术的60 h以上缩短为30 h以内，乙醇浓度由5%～6%（v/v）平均提高到10.69%（v/v），最高可达12.41%（v/v），发酵效率由88%以下提高至90%以上，可提高单位设备的生产力，降低乙醇蒸馏能耗，节能效果明显。

关键词：燃料乙醇 粘度 非粮

Abstract：In terms of the mechanism of viscosity， sweet potato was used as a model material and investigated with polysaccharide monoclonal antibody microarray. The results of this analysis explained what kinds of component related with viscosity and provided targets for viscosity reduction technology. Complex enzymes was developed for viscosity reduction， and fungi which could produce complex viscosity reduction enzymes was screened. Under the function of these enzymes， viscosity of sweet potato，cassava and canna was reduced by 90%. In terms of microbe， 8 strains of yeast were screened， and then acclimation to high temperature and high concentration of ethanol was carried out. The mechanism of stress tolerance of these yeasts was investigated， and the results would be benefit to better performance of these yeasts. In terms of fermentation technology， one fermentation stimulant was developed. Under the function of this stimulant，12% ethanol could be produced within 24h. In terms of reactor， novel reactor with high performance and low energy consumption was developed， which could blend high viscosity sweet potato mash well. After system integration， these technologies were applied to 30，000t scale of production line in the biggest ethanol plants in southwest of China. Fermentation time was reduced from more than 60h to less than 30h， ethanol concentration was increased from 5%～6% to 12%， and ferment efficiency was enhanced from 88% to 90%. These changes of parameters could enhance productivity of facilities， and decrease energy consumption significantly.

Key Words：Fuel Ethanol；Viscosity；Non-grain Material

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