Expression of mucin regulated by recombinant human interleukin-6 in HT-29 cell of colon cancer

2012-08-15 00:54:32HuangHuaming
外科研究与新技术 2012年1期
关键词:华明

/Huang Huaming

(黄华明,Dept Gen Surg,1st Affil Hosp Sanxia Univ,Hubei Yichang 430002)…∥Chin J Gastrointest Surg.-2011,14(8).-899 ~902

ObjectiveTo study the regulation of the mucin protein in colon cancer cell line HT-29 by recombinant human interleukin-6(rIL-6)and to further elucidate the development of colon caner.MethodsThe HT-29 cells were treated with different concentrations of rIL-6(1,2,5,10 and 20 μg/L),then flow cytometry and RT-PCR were used to detect the expression of mucin l and mucin 2.Transwell invasion assay was used to observe the effect of invasion capability of rIL-6 to HT-29 cells.ResultsIn colon cancer,the expression of mucin 1 could be promoted by rIL-6 with concentration above 2 μg/L,the expression rates were(12.5 ± 1.6)%,(26.6 ± 2.7)%,(33.9 ±2.8)%and(58.9 ±2.5)%,respectively,higher than(8.0 ± 0.8)%in the negative controls(P <0.01),meanwhile,the expression of mucin 2 decreased by rIL-6 with concentration above 2 μg/L,the expression rates were(30.5 ±2.6)%,(17.0 ±2.7)%,(11.0 ±2.0)%and(5.3 ± 1.8)%,respectively,lower than(41.6 ± 3.6)%in negative control(P < 0.01).With the increase in rIL-6 concentration,the invasion of HT-29 cells was enhanced.ConclusionIn colon cancer,the expression of mucinl can be promoted by rIL-6,while the expression of mucin2 can be inhibited.IL-6 is a promoting effect factor in colon cancer invasion and metastasis.12 refs,3 figs,2 tabs.

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